在动物进化之前,SPIRE活性核子的作用是Actomyosin有机体的功能
Martin Kollmar1, Tobias Welz2, Aishwarya Ravi3
1Group Systems Biology of Motor Proteins, Department of NMR-based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany. mako@mpinat.mpg.de.
Communications biology
|July 8, 2024
概括
SPIRE 活性蛋白核子发生在早期的动物祖先中. 这些蛋白与RAB GTPases,formins和myosin电机相互作用,使有机体运输成为可能,并可能有助于多细胞进化.
科学领域:
- 细胞生物学 细胞生物学
- 进化生物学 进化生物学
- 生物化学 生物化学
背景情况:
- 细胞骨蛋白质驱动细胞结构和功能进化.
- 斯派尔 (SPIRE) 激素核子发生器的进化起源和保存状况尚不清楚.
- 哺乳动物SPIREs通过actin纤维,RAB GTPases,formins和myosin电机促进有机细胞的运输.
研究的目的:
- 为了研究SPIRE活性核子核子的进化起源.
- 为了确定跨物种的SPIRE蛋白的功能性保护.
- 探索SPIRE在多细胞进化的作用.
主要方法:
- 在真核生物中进行序列搜索,以识别SPIRE同类.
- 生物化学试验测试SPIRE,RAB GTPases,formins和myosin之间的相互作用.
- 在哺乳动物系统中的单细胞全生动物中使用SPIRE进行功能补充测试.
- 孔焦显微镜用于观察类鞭毛动物中的蛋白质定位.
主要成果:
- 在全生动物中发现了SPIRE蛋白,但在其他真核生物中没有.
- 来自单细胞片体的SPIRE与RAB,formin和肌蛋白相互作用.
- 这种鞭状SPIRE核化了活性丝,并拯救了哺乳动物在有机体运输中的SPIRE功能.
- SPIRE和肌酸蛋白在Salpingoeca rosetta中的细胞器上同位.
结论:
- 蜘蛛蛋白可能起源于单细胞全息动物的祖先.
- SPIRE促进了一种基于actin-myosin的外细胞运输机制.
- 这种古老的运输系统可能对复杂的多细胞动物的进化至关重要.
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