相关实验视频

Updated: Jun 21, 2025

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

Published on: March 11, 2018

饮食限制促进基于转录组分析的老的精子重塑

Wenjie Liang¹, Yuehua He¹, Tingqi Zhu¹

¹College of Animal Science and Technology, Henan Agricultural University, Ping'an Avenue 218#, Zhengdong New District, Zhengzhou, 450046, P. R. China.

|July 8, 2024

概括

诱导的子改善了精子质量和丸重量. 转录基因组测序确定了61个差异表达的基因,包括WNT5A和FGFR3,有可能提高老化的生育能力.

科学领域:

禽畜科学禽畜科学禽畜科学
生殖生物学生殖生物学
基因组学就是基因组学.

背景情况:

老化繁殖的生育能力下降,影响家禽生产.
提高老的生育能力对于延长它们的生产寿命至关重要.
目前提高公生育能力的方法面临着重大挑战.

研究的目的:

为了确定与老年胡丹的精子改造相关的基因.
使用转录组测序分析精液质量和丸发育之间的相关性.
提供数据,以提高老年的繁殖价值.

主要方法:

挑选的350天大的胡丹公经过诱导化 (D组) 和非诱导化 (47DG组).
在丸组织上进行转录组测序 (RNA-seq),以分析差异表达基因 (DEG).
使用定量实时PCR (qPCR) 来验证选定的DEGs的表达.

主要成果:

诱导造显著增加了精子的运动性,精子密度和子的体重.
在组之间,RNA-seq确定了61个DEG (21个上调,40个下调).
DEGs在包括细胞因子-细胞因子受体相互作用,Wnt信号传递和MAPK信号传递在内的途径中得到丰富.
六个基因 (WNT5A,FGFR3,AGTR2,TGFβ2,ROMO1,SLC26A7) 被确定为可能在丸发育和精子生成中发挥作用.

结论:

诱导造对老的精子质量和丸发育有积极的影响.
确定的特定基因可能是提高生育能力的关键目标.
这项研究提供了基础数据,以提高老化家禽的繁殖效率.

关键词:

化化化生殖表现的表现生殖表现的表现子子子精子发生是精子生成.转录基因组.转录基因组.一个转录基因组.

更多相关视频

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Published on: November 26, 2017

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Sperm Collection of Differential Quality Using Density Gradient Centrifugation

Published on: November 29, 2018

相关概念视频

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...