大规模集成的共同表达分析揭示了酵母非正规的翻译组的转录调节,进化和细胞影响
April Rich1,2,3, Omer Acar1,2,3, Anne-Ruxandra Carvunis4,5
1Joint Carnegie Mellon University-University of Pittsburgh, University of Pittsburgh Computational Biology PhD Program, University of Pittsburgh, Pittsburgh, PA, USA.
Genome biology
|July 8, 2024
概括
成千上万的非正典的开放阅读框架 (nORFs) 被转录和翻译. 这项研究开发了一个分析nORF联合表达的框架,揭示了它们在细胞运输和平衡中的作用,以及它们如何利用基因促进剂.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 酵母生物学的酵母生物学
背景情况:
- 成千上万的非正规的开放阅读框架 (nORF) 在注释基因之外被转录和翻译.
- 推断nORF对细胞表型的贡献是具有挑战性的,因为它们的长度很短,起源很近,表达率很低.
- 传统的方法不足以研究nORFs.
研究的目的:
- 研究Saccharomyces cerevisiae中的nORFs的转录调节,进化和细胞作用.
- 开发一个共同表达分析框架,以考虑低表达水平.
- 为了解nORF集成到蜂网络提供资源.
主要方法:
- 开发了一个专门的共表达分析框架,适用于低表达数据.
- 分析了酵母中的nORFs的转录调节和同表达模式.
- 使用Web浏览器界面进行数据查询和可视化.
主要成果:
- nORFs优先与参与细胞运输和恒温的基因共同表达.
- 年轻的de novo nORFs通过转录阅读利用邻近的基因促进体,增强共表达和表达.
- "转录背"影响nORF的共同表达,但并不总是表达水平.
- 大约40%的nORFs形成了独立于已知的基因的独特的转录模块.
- 有一个网页界面可用于访问共表达数据.
结论:
- nORF转录是一个高度规范的过程.
- 开发的协同表达数据集是研究nORF功能,细胞集成和进化的宝贵资源.
- nORF在细胞网络中发挥作用,并对细胞表型作出贡献.
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