Jove
Visualize
联系我们

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Does insulin bolster antioxidant defenses via the extracellular signal-regulated kinases-protein kinase B-nuclear factor erythroid 2 p45-related factor 2 pathway?

Antioxidants & redox signaling·2011
Same author

Decrease in calcium-sensing receptor in the progress of diabetic cardiomyopathy.

Diabetes research and clinical practice·2011
Same author

JAMIE: A software tool for jointly analyzing multiple ChIP-chip experiments.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Morphine-induced conditioned place preference in mice: metabolomic profiling of brain tissue to find "molecular switch" of drug abuse by gas chromatography/mass spectrometry.

Analytica chimica acta·2011
Same author

[The interventions effect-assessment of the workers exposed to N, N-dimethylformamide by percutaneous in a synthetic leather factory].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2011
Same author

[The analysis of effect of Th1/Th2 cytokine in the different prognosis in severe influenza A (H1N1)].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology·2011
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 21, 2025

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K

在scRNA-seqq中监督细胞类型识别的目标导向参考构造.

Xin Wei1, Wenjing Ma2, Zhijin Wu1

  • 1Brown University.

Research square
|July 9, 2024
PubMed
概括

在单细胞RNA测序 (scRNA-seq) 中监督细胞类型识别受益于一种名为目标导向参考构造 (TORC) 的新策略. 通过创建针对目标数据量身定制的更好的参考数据集,TORC提高了准确性.

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 准确的细胞类型识别对于单细胞RNA测序 (scRNA-seq) 数据分析至关重要.
  • 监督方法提供高精度和效率,但严重依赖于参考数据集质量.
  • 现有的工具缺乏标准化的方法来选择和构建最佳的参考数据集.

研究的目的:

  • 引入面向目标的参考构造 (TORC),这是一个用于在scRNA-seq.中构建参考数据集的新策略.
  • 为了应对数据分布和基准数据集和目标数据集之间的细胞类型组成差异的挑战.
  • 为了提高在scRNA-seq.中监督细胞类型识别的性能.

主要方法:

  • 托克是构建参考数据集的广泛适用的策略.
  • 该方法特别针对基于给定的目标数据集构建引用.
  • TORC的目标是缓解数据分布和细胞类型组成的差异.

主要成果:

  • 对模拟和真实scRNA-seq数据进行了广泛的基准测试.
  • 在TORC中,细胞类型识别准确度得到了持续的改善.
  • 该战略在调整参考数据和目标数据特征方面被证明是有效的.
关键词:
细胞类型识别 细胞类型识别参考建筑的参考建筑.监督学习学习 监督学习这就是scRNA-seqq.

更多相关视频

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.4K

相关实验视频

Last Updated: Jun 21, 2025

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

13.8K
Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
10:12

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

18.5K
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
11:34

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

Published on: July 18, 2019

16.4K

结论:

  • 在监督scRNA-seq分析中,TORC为参考数据集构建提供了强大的解决方案.
  • 该方法提高了细胞类型识别的可靠性和准确性.
  • TORC是免费的,促进其在研究界的采用.