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生物信息学和系统生物学方法用于识别COVID-19感染和肉类的潜在常见病原体
Jun Zhong1, Hui Yuan1, Jinghong Yang1
1School of Clinical Medicine, Southwest Medical University, Luzhou, Sichuan, China.
萨科佩尼亚和COVID-19共享共同的途径和枢纽基因,表明潜在的治疗点. 这项研究确定了用于诊断这两种疾病的ALDH1L2和KLF5等关键基因.
科学领域:
- 基因组学和生物信息学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 肉症是一种与年龄相关的肌肉质量和力量的损失,与COVID-19感染率的增加和更糟糕的结果有关.
- 连接缩症和2019年新冠肺炎 (COVID-19) 的潜在分子机制在很大程度上是未知的.
- 了解共享途径对于开发针对COVID-19的形患者的向疗法至关重要.
研究的目的:
- 通过使用公开的高通量数据,在sarcopenia和COVID-19之间识别常见的差异表达基因 (DEGs).
- 阐明共享的致病途径和潜在的关键调节基因和分子.
- 为了评估被识别的基因的诊断潜力,对sarcopenia和COVID-19进行评估.
主要方法:
- 来自GEO数据库的肉症 (GSE111016) 和COVID-19 (GSE171110) 的基因表达数据的比较分析.
- 识别常见的DEG,枢纽基因 (使用cytoHubba) 和通路丰富分析 (GO,KEGG).
- 调控网络的构建 (TF基因,miRNA基因),基因疾病关联分析,免疫细胞透评估 (CIBERSORT) 和ROC曲线分析.
主要成果:
- 鉴定出66种常见的DEGs (34个上调,32个下调) 和15个枢纽基因.
- 发现了sarcopenia和COVID-19之间的共同功能和途径;FOXOC1和hsa-mir-155-5p被确定为潜在的关键调节者.
- 通过ROC分析,ALDH1L2和KLF5基因对这两种情况都显示出显著的诊断准确性;免疫细胞透与枢纽基因表达相关.
结论:
- 萨科佩尼亚和COVID-19可能共享共同的潜在病原性途径.
- 已识别的枢纽基因和监管网络为治疗策略提供了新的目标.
- ALDH1L2和KLF5代表了与COVID-19并发的萨尔科佩尼亚和COVID-19的有希望的诊断生物标志物.
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