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在增殖细胞中静态基因表达:在单细胞和人群视角中不同噪声强度
Zhanhao Zhang1, Iryna Zabaikina2, César Nieto1
1Department of Electrical and Computer Engineering, University of Delaware. Newark, DE 19716, USA.
bioRxiv : the preprint server for biology
|July 9, 2024
概括
基因表达噪声在单细胞和群体之间存在差异. 积极的反在人群中更大程度上放大了噪音,可能低估了基因产品的变异性.
科学领域:
- 系统生物学 系统生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 基因表达噪声对于细胞功能和适应至关重要.
- 对于研究噪音存在两个主要框架:单细胞时间缩短和人口快照.
- 这些框架之间的差异可能来自细胞过程,如生长和分裂.
研究的目的:
- 系统地调查单细胞和人群噪声测量分歧的场景.
- 分析正反循环和分子爆发对基因表达噪声的影响.
- 评估细胞周期变化和分子分离如何影响噪声估计.
主要方法:
- 开发和分析一个包含分子爆破的随机模型.
- 对蛋白质度的稳定状态分布的分析预测.
- 在不同条件下,从单细胞和群体视角得出的噪声水平的比较.
主要成果:
- 积极的反循环在人口框架中比在单细胞框架中更显著地放大基因表达噪声.
- 从人口的角度来看,随机分离过程中分子分裂的噪音被细胞周期持续时间的变化放大.
- 单细胞观点,当不考虑增殖时,可能会低估真正的基因产品噪声水平.
结论:
- 框架的选择显著影响基因表达噪声的评估.
- 基于人群的噪音测量可能对某些生物过程 (如反和细胞分裂) 更敏感.
- 这些发现对于理解应激反应和增长抑制途径的细胞间变异至关重要.
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