相关实验视频
Updated: Jun 20, 2026
06:56
Isolation of Double Negative αβ T Cells from the Kidney
Published on: May 16, 2014
12.3K
结直肠癌中的双阴性T细胞及其相应的淋巴结的特征
Kazumi Okamura1, Lifang Wang1, Satoshi Nagayama2,3
1Project for Immunogenomics, Cancer Precision Medicine Center, Japanese Foundation for Cancer Research, Tokyo, Japan.
Oncoimmunology
|July 9, 2024
概括
双阴性T细胞 (DNT) 在结直肠瘤中被丰富,可能来自CD8+T细胞. 这些DNT细胞可能通过失去细胞毒性功能来抑制抗瘤免疫力.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 双阴性T (DNT) 细胞 (TCRαβ+ CD4- CD8-) 在周围血液中罕见,已知在炎症和自身免疫中起作用.
- 它们在瘤微环境中的功能基本上是未知的.
研究的目的:
- 研究DNT细胞在结直肠癌 (CRC) 组织和瘤排水淋巴结 (TDLNs) 的特征,起源和功能.
主要方法:
- 在CRC组织和TDLN中对DNT细胞群的比较分析.
- 测序T细胞受体 (TCR) 来推断DNT细胞的起源.
- 单细胞转录组分析以表征DNT细胞表型和基因表达.
- 流式细胞计量以评估细胞毒性介质的产生.
主要成果:
- 与TDLN相比,DNT细胞在CRC组织中被显著丰富,特别是在T细胞透率低的瘤中.
- TCR测序表明,DNT细胞的一个子集的CD8+T细胞起源.
- 单细胞分析显示DNT细胞群类似于中央记忆中的CD8+T细胞,具有高GZMK表达,但低效应/耗尽标记.
- DNT细胞表现出减少细胞毒媒介的产生.
结论:
- DNT细胞在结直肠瘤微环境中被丰富,可能来自CD8+ T细胞.
- 这些DNT细胞表现出独特的表型和降低的细胞毒性功能,表明它们可能充当抗瘤免疫反应的负调节者.
更多相关视频
10:29Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Published on: August 14, 2019
10.6K
07:45Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis
Published on: January 26, 2024
1.9K
相关概念视频
01:18Lymphoid Cells and Tissues
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...