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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jun 21, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

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改进了定量单分子显微镜的成像表面.

Yu P Zhang1,2, Evgeniia Lobanova1,2, Asher Dworkin1,2

  • 1Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

ACS applied materials & interfaces
|July 9, 2024
PubMed
概括
此摘要是机器生成的。

一个新的RainX-F127 (RF-127) 表面在单分子显微镜中显著降低了非特异性结合. 这种改进的被动化可以在各种生物流体中灵敏地检测蛋白质聚合物.

关键词:
绘制图像的表面表面.蛋白质聚合物是一种蛋白质聚合物.单分子显微镜的显微镜.超分辨率显微镜的显微镜.表面化学 表面化学表面被动化 表面被动化

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科学领域:

  • 生物物理学的生物物理.
  • 材料科学 材料科学 材料科学
  • 生物化学 生物化学

背景情况:

  • 非特定的结合阻碍了基于表面的单分子显微镜中的敏感检测.
  • 现有的被动化方法,如聚乙烯糖醇 (PEG) 表面,在防止聚合物结合方面存在局限性.

研究的目的:

  • 为定量单分子显微镜开发一种具有增强被动化的简化表面.
  • 与传统表面相比,减少蛋白质聚合物的非特异性结合.

主要方法:

  • 简化的RainX-F127 (RF-127) 表面的制造.
  • 对RF-127表面被动化与蛋白质聚合物的评估.
  • 测试与SiMPull和超分辨率成像等单分子技术的兼容性.
  • 在各种生物流体中检测alpha-synuclein (α-syn) 和tau聚合物.

主要成果:

  • 与PEG表面相比,RF-127表面的非特异性结合率高达100倍.
  • 表面允许在血清,大脑提取物,脑脊液和唾液中特定检测α-syn和tau聚合物.
  • 该方法证明与各种单分子测试兼容,并允许微板功能化.

结论:

  • 简化的RF-127表面为定量单分子显微镜提供了一个强大而通用的平台.
  • 这种改进的表面增强了在多种生物流体类型中检测蛋白质聚合物的灵敏度和特异性.
  • 该方法是可访问的,不需要专门的设备,并促进高通量应用.