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Updated: Jun 21, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

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Published on: October 31, 2015

改进了定量单分子显微镜的成像表面

Yu P Zhang^1,2, Evgeniia Lobanova^1,2, Asher Dworkin^1,2

¹Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.

ACS applied materials & interfaces

|July 9, 2024

概括

一个新的RainX-F127 (RF-127) 表面在单分子显微镜中显著降低了非特异性结合. 这种改进的被动化可以在各种生物流体中灵敏地检测蛋白质聚合物.

科学领域:

生物物理学的生物物理.
材料科学材料科学材料科学
生物化学生物化学

背景情况:

非特定的结合阻碍了基于表面的单分子显微镜中的敏感检测.
现有的被动化方法,如聚乙烯糖醇 (PEG) 表面,在防止聚合物结合方面存在局限性.

研究的目的:

为定量单分子显微镜开发一种具有增强被动化的简化表面.
与传统表面相比,减少蛋白质聚合物的非特异性结合.

主要方法:

简化的RainX-F127 (RF-127) 表面的制造.
对RF-127表面被动化与蛋白质聚合物的评估.
测试与SiMPull和超分辨率成像等单分子技术的兼容性.
在各种生物流体中检测alpha-synuclein (α-syn) 和tau聚合物.

主要成果:

与PEG表面相比,RF-127表面的非特异性结合率高达100倍.
表面允许在血清,大脑提取物,脑脊液和唾液中特定检测α-syn和tau聚合物.
该方法证明与各种单分子测试兼容,并允许微板功能化.

结论:

简化的RF-127表面为定量单分子显微镜提供了一个强大而通用的平台.
这种改进的表面增强了在多种生物流体类型中检测蛋白质聚合物的灵敏度和特异性.
该方法是可访问的,不需要专门的设备,并促进高通量应用.

关键词:

绘制图像的表面表面.蛋白质聚合物是一种蛋白质聚合物.单分子显微镜的显微镜.超分辨率显微镜的显微镜.表面化学表面化学表面被动化表面被动化

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