相关实验视频

Updated: Jun 21, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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由II型限制-修改系统介导的分离后杀戮的依赖性取决于限制内核酶有效活动的寿命

Svetlana Kozlova1, Natalia Morozova2, Yaroslav Ispolatov3

  • 1Skolkovo Institute of Science and Technology, Center for Molecular and Cellular Biology, Moscow, Russia.

mBio
|July 9, 2024
PubMed
概括

通过限制修饰 (RM) 系统进行分离后杀死 (PSK) 取决于酶活性寿命. 大肠杆菌中的CRISPR干扰表明,只有当限制性内核酶活性在两个细胞分裂周期内持续时,PSK才会发生.

关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.塑的稳定性 塑的稳定性在隔离后的杀戮.限制 修改 限制 修改

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