一个由乙酸依赖的修饰网络调节了在Actinobacteria中的c-di-AMP稳态
Yu Fu1, Liu-Chang Zhao1, Jin-Long Shen1
1Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
乙酸盐 (AcP) 通过乙化调节蛋白质DisA和DasR来控制循环二氨酸单酸盐 (c-di-AMP) 稳态. 这种机制影响细菌生长,细胞生理学和对环境信号的反应差异化.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 微生物学 微生物学
背景情况:
- 循环纯氨酸核酸,如3',5'-循环二-腺单酸盐 (c-di-AMP),是所有生命形式的关键信号转导分子.
- 控制细胞内c-di-AMP水平和平衡的精确信号和分子机制在很大程度上仍未定义.
研究的目的:
- 阐明控制c-di-AMP稳态的监管网络.
- 确定乙酸 (AcP) 在调节c-di-AMP水平中的作用.
- 在这个过程中调查二甲基酸环酶DisA和转录抑制剂DasR的功能.
主要方法:
- 在Saccharopolyspora erythraea*中研究了AcP,DisA和DasR之间的相互作用.
- 分析了AcP诱导的乙化对DisA和DasR活性和多元化的影响.
- 研究了这些修改对c-di-AMP合成和*disA*基因表达的影响.
主要成果:
- 在K66的AcP诱导的DisA的乙化抑制其循环酶活性,减少c-di-AMP的合成.
- 在K78的DasR的乙化缓解了*disA*的转录抑制,增加了c-di-AMP水平.
- AcP充当分子开关,通过DisA和DasR的翻译后修饰来平衡c-di-AMP稳态.
结论:
- 通过AcP介导的DisA和DasR的乙化是维持c-di-AMP恒温的关键机制,以应对环境信号.
- 这些乙化事件诱导了对细菌分化至关重要的形状变化.
- 这种调节途径代表了Actinobacteria*细胞控制的基本方面.
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