对比转录组学揭示了一种新型的晚年特异性DNA结合蛋白,该蛋白因对电离辐射的反应而诱导
Marwan Anoud1,2, Emmanuelle Delagoutte1, Quentin Helleu1
1Département AVIV, MNHN, CNRS UMR7196, INSERM U1154, Paris, France.
eLife
|July 9, 2024
概括
由于高效的DNA修复,尾动物能够抵抗极端辐射. 一个新型的基因,Tardigrade DNA损伤反应1 (TDR1),通过与DNA相互作用来增强这种放射性抵抗.
科学领域:
- * 分子生物学 * 分子生物学
- * 辐射生物学 辐射生物学
- * 遗传学 遗传学是一门学科
背景情况:
- * 晚级动物对电离辐射 (IR) 具有显著的抵抗力.
- * 了解落后级放射电阻背后的分子机制对于生物和医学应用至关重要.
- *DNA损伤和修复途径是细胞在压力下生存的核心.
研究的目的:
- * 为了研究暴露于红外线的迟行动物的DNA双和单链断裂诱导和修复.
- * 通过比较转录组学来识别有助于放射电阻的新型晚年动物特异基因.
- *为了描述一个新发现的基因的功能,Tardigrade DNA损伤反应1 (TDR1).
主要方法:
- * 对*Hypsibius exemplaris*暴露于电离辐射,以表征DNA损伤和修复.
- *对三种迟行物种进行比较转录组学分析,以确定差异表达的基因.
- *蛋白与蛋白相互作用研究和人类细胞中的表达,以评估TDR1功能.
主要成果:
- * 晚级动物的DNA单链断裂诱导与人类细胞相当,这凸显了DNA修复的重要性.
- *比较的转录组学揭示了DNA修复基因的过度表达和一种新的晚年特异基因,TDR1.
- * TDR1蛋白与DNA相互作用,形成聚合物,并在人类细胞中表达时增强放射电阻.
结论:
- * DNA 修复机制对于晚期放射电阻至关重要.
- * TDR1是一种新型的晚年特异基因,在对DNA损伤的反应中起着重要作用.
- *TDR1在改善细胞对辐射和基因毒剂的抵抗方面具有潜在的应用.
相关概念视频
Transcription Attenuation in Prokaryotes
15.2K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.2K
Translesion DNA Polymerases
9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K
General Transcription Factors
5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Transcription Factors
75.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.8K


