相关实验视频

Updated: Jun 21, 2025

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Human Circadian Phenotyping and Diurnal Performance Testing in the Real World

Published on: April 7, 2020

在循环中的生物上散布一些雾:测量,生理间隔和病理生理学含义

Hai-Bo Wang¹, Xuan Xiao¹, Wen Dai¹

¹Department of Clinical Laboratory, Institute of Translational Medicine, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Metabolomics : Official journal of the Metabolomic Society

|July 9, 2024

概括

这项研究引入了一种新方法,用于精确测量血中的生物素 (BH4,BH2). 它建立了生理范围,并确定了生物水平与健康指标 (如年龄和高尿血) 之间的联系.

科学领域:

生物化学生物化学
分析化学分析化学
临床诊断临床诊断临床诊断

背景情况:

生物素,包括四生物素 (BH4),二生物素 (BH2) 和生物素 (B),是重要的酶辅因子.
目前对生物蛋白的研究面临着挑战,原因是分析限制和不清楚的生理范围.
了解生物的代谢对于各种临床应用至关重要.

研究的目的:

开发和验证一种新的分析方法,用于在血中进行全面的生物蛋白分析.
在健康个体中建立血生物的生理参考间隔.
通过将其与生物化学指标相关联,研究生物水平的病理生理学影响.

主要方法:

开发了一种新的液体染色学-双重质谱法 (LC-MS/MS) 方法,包括化学衍生和冷诱导相分离.
来自看似健康的个体的血样本被分析以确定生理范围.
进行了统计分析,以探索生物水平与临床参数之间的相关性.

主要成果:

开发的LC-MS/MS方法证明了在整个生理范围检测生物的可靠性,强调了需要及时进行抗氧化.
报告了BH4,BH2,BH4/BH2比率的确定的生理范围,以及总生物质蛋白.
在生物的状态和诸如年龄,性别,高尿血和超重条件等因素之间观察到显著的相关性.

结论:

新的LC-MS/MS方法,与适当的抗氧化,允许准确的血生物评估.
建立的生理间隔和确定的病理生理相关性为未来的临床研究提供了基础.
这项研究增强了对生物在人类健康和疾病中的作用的理解.

关键词:

生物素是一种生物素.这是一种二氧化二氧化.质谱测量质量谱测量氧化应激是一种氧化应激.水生物二烯 (Tetrahydrobiopterin) 是一种一种

更多相关视频

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Published on: January 19, 2017

Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy

Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy

Published on: February 20, 2018

相关概念视频

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...

Pharmacokinetic Models: Comparison and Selection Criterion

Pharmacokinetic Models: Comparison and Selection Criterion

Physiological and compartmental models are valuable tools used in studying biological systems. These models rely on differential equations to maintain mass balance within the system, ensuring an accurate representation of the dynamic processes at play.
Physiological models take a detailed approach by considering specific molecular processes. They can predict drug distribution, metabolism, and elimination changes, providing a comprehensive understanding of how drugs interact with the body.

Measurement of Blood Pressure

Measurement of Blood Pressure

Assessing blood pressure is a standard procedure executed in virtually all medical environments. The method utilized today was established over a hundred years ago by an innovative Russian doctor, Dr. Nikolai Korotkoff. The soft ticking noise, known as Korotkoff sounds, heard while taking blood pressure readings results from turbulent blood flow within the vessels. The apparatus required for this procedure includes a sphygmomanometer, a blood pressure cuff attached to a gauge, and a...

Factors Affecting Drug Biotransformation: Biological

Factors Affecting Drug Biotransformation: Biological

Biological factors significantly impact drug metabolism, influencing drug clearance, efficacy, and potential toxicity.
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...