用Limosilactobacillus fermentum (OR553490) 生物合成的纳米颗粒的抗候性应用
Esraa Ali Mohamed1, Mohamed Marzouk El-Zahed2
1Department of Botany and Microbiology, Faculty of Science, Damietta University, New Damietta, 34517, Egypt.
Discover nano
|July 9, 2024
概括
研究人员开发了一种新的,具有成本效益的方法,使用有益细菌制造纳米粒子 (Se NPs). 这些SeNP有效地对抗耐药Candida albicans感染,毒性最小,提供了一个有前途的新疗法策略.
科学领域:
- 纳米技术 纳米技术
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 虫是一种危险的病原体,对抗真菌药物的耐药性越来越强.
- 对于克服耐药性的新型抗候群药物有着至关重要的需求.
研究的目的:
- 为纳米粒子 (Se NPs) 开发一种简单,具有成本效益和安全的生物合成方法.
- 评估合成的Se NPs对Candida albicans的抗候群活性和细胞毒性.
主要方法:
- 使用Limosilactobacillus发酵素和烯酸盐的无细胞超剂,对Se NP进行生物合成.
- 优化Se NPs合成条件 (混合比,时间,温度).
- 使用UV-Vis,FT-IR,TEM,XRD,Zeta潜力和EDS分析对Se NP的表征.
- 对C. albicansATCC10231进行抗菌活性测试和对角质细胞进行细胞毒性评估.
主要成果:
- 优化的Se NPs合成产生了晶体,分散良好的纳米粒子 (17-30 nm),吸收峰值在254 nm.
- 来自L. fermentum的蛋白质作为稳定剂和封闭剂,由FT-IR证实.
- 与米可纳相比,Se NPs表现出较强的C. albicans抑制 (26毫米区域,15微克/毫升MIC)
- 对于正常的角质细胞 (IC50 ≈ 41.5 微克/毫升),Se NPs 的细胞毒性较低.
结论:
- 使用L. fermentum的SeNP的绿色合成是一种可行的和高效的方法.
- 合成的Se NPs显示出强大的抗体活性和有利的安全性.
- 这种方法提供了一个有希望的新策略,用于开发抗真菌疗法,以对抗耐药C. albicans.
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