相关实验视频

Updated: Jun 21, 2025

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

依赖GRP75的线粒体-ER接触确保了小鼠小细胞早期发育过程中的细胞存活

Fan Zhao¹, Zejin Cui², Pengfei Wang³

¹Institute of Immunology and Department of Rheumatology at Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.

Developmental cell

|July 9, 2024

概括

线粒体和内质网膜接触 (MERC) 对T细胞发育至关重要. 破坏GRP75,一个关键的MERC蛋白质,通过触发线粒体应激和免疫反应来阻止T细胞成熟.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
发展生物学发展生物学

背景情况:

线粒体和内质网膜接触 (MERCs) 调节重要的细胞功能,如生存和分化.
在小胞体发育的CD4-CD8双阴性 (DN) 阶段,MERCs特别丰富.

研究的目的:

为了研究GRP75介导的MERCs在小鼠早期乳腺细胞发育中的作用.
阐明GRP75在T细胞发育中的功能背后的分子机制.

主要方法:

在小鼠中对T细胞特异的Hspa9 (编码为GRP75) 的淘汰.
在DN3-DN4过渡期对胸细胞活力和发育进展的分析.
评估线粒体应激,线粒体DNA释放,I型干扰素 (IFN-I) 反应和脂质过氧化 (LPO).

主要成果:

特定于T细胞的Hspa9淘汰损害了DN3小细胞的活力,并在DN3-DN4阶段导致发育停止.
GRP75 缺乏导致线粒体压力,线粒体DNA (mtDNA) 的细胞质释放,以及I型干扰素 (IFN-I) 途径的激活.
确定IFN-I通路和下游脂质过氧化 (LPO) 是细胞存活能力受损和发育阻塞的关键因素.

结论:

依赖GRP75的MERC在提摩细胞早期发育中起着至关重要的作用.
GRP75-MERCs轴在T细胞发育的DN阶段控制细胞生存和分化.

关键词:

在 GRP75 中,GRP75 是国际货币基金组织-I 细胞存活率细胞存活率线粒体与ER接触甲状腺细胞的发育.

更多相关视频

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

相关概念视频

Mitochondrial Precursor Proteins

Mitochondrial Precursor Proteins

Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70 chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...

Translocation of Proteins into the Mitochondria

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...

Mitochondrial Protein Sorting

Mitochondrial Protein Sorting

Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...