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相关概念视频

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

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The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
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Histone Modification02:32

Histone Modification

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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
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Histone Variants at the Centromere02:30

Histone Variants at the Centromere

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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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Spreading of Chromatin Modifications02:25

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
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Co-activators and Co-repressors02:04

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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Heterochromatin02:38

Heterochromatin

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The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
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Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter
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CoREST复合体调节了多个质子的时间修饰,特别是在时钟神经元中.

Pengfei Lv1, Zhangwu Zhao1, Yukinori Hirano2

  • 1Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, People's Republic of China.

Open biology
|July 9, 2024
PubMed
概括

元素的共同抑制器 (CoREST) 复合体通过改变时钟神经元中周期 (Per) 位点的基因组修饰来调节昼夜节律. 这种表观遗传调节会影响每次转录和昼夜时间.

关键词:
这就是COREST.这种植物是Drosophila.时间周期 时间周期昼夜节律 昼夜节律基斯顿基因基因基因基因基因基因基因基因基因基因基因基因基因

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科学领域:

  • 时间生物学 时间生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学

背景情况:

  • 循环节律依赖于表观遗传调节,在周期 (Per) 位点观察到的基因组修饰.
  • 以前的研究往往没有关注时钟神经元,这是生物节律调节的关键组成部分.

研究的目的:

  • 调查元素的共同抑制器 (CoREST) 复合体在昼夜节律调节中的作用.
  • 为了确定CoREST是否影响Per转录和相关的质子修饰,特别是在时钟神经元内.

主要方法:

  • 利用COREST突变屏幕来识别对昼夜节律的影响.
  • 进行了基因和物理相互作用测试,以探索调节关系.
  • 在时钟神经元中进行了组织特异性染色质免疫沉,以分析Per locus中的组质子修饰.

主要成果:

  • 一个COREST突变通过影响Per转录导致昼夜节律缺陷.
  • 在Perlocus.中,CoREST与基因素修饰剂相互作用.
  • 在时钟神经元中的Per locus中,CoREST突变诱导了基因组修饰的时间依赖性变化.

结论:

  • CoREST复合体在通过时钟神经元中的表观遗传机制调节昼夜节律方面发挥着至关重要的作用.
  • 这项研究强调了Per locus的动态表观遗传变化及其在昼夜基因表达中的重要性.