BcWRKY1通过抑制JA生物合成来赋予Botrytis cinerea的敏感性
Shuilin Yuan1,2, Yuan Wang1, Die Hu1,3
1National Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Physiologia plantarum
|July 9, 2024
概括
这项研究表明,BcWRKY1对中国白菜对Botrytis cinerea的抗病能力有负面调节. 沉默BcWRKY1通过调节活性氧物种和莉酸通路来增强植物免疫力.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 植物病理学 植物病理学
背景情况:
- WRKY转录因子对于植物应激反应至关重要.
- WRKYs在无标题中国白菜 (Brassica campestris ssp. 虫 (Chinensis) 对虫 (Botrytis cinerea) 感染的情况基本上是未知的.
研究的目的:
- 为了阐明BcWRKY1在Botrytis cinerea感染期间在非标题中国白菜中的功能.
- 研究BcWRKY1介导的抗病性基因的分子机制.
主要方法:
- 植物中的基因沉默和过度表达 (非标题中国菜和阿拉比多普西斯).
- 测量反应性氧物种 (ROS) 和莉酸 (JA) 水平.
- 对基因表达 (BcOPR3,BcLOX3-1,BcLOX3-2) 和促进体活性 (W-box) 的分析.
- 酵母二杂交 (Y2H) 选,以确定相互作用的蛋白质.
主要成果:
- BcWRKY1的表达是由B. cinerea引起的.
- 抑制BcWRKY1增强了抵抗力,减少了ROS,增加了JA含量和相关的基因表达.
- BcWRKY1通过准W盒直接抑制了BcLOX3-2转录.
- BcWRKY1与BcCaM6相互作用,这种相互作用减弱了BcLOX3-2.6的抑制.
结论:
- BcWRKY1作为非标题中国白菜对B. cinerea的抗病性负调节剂.
- BcWRKY1-BcCaM6复合体在调节植物防御通路方面发挥作用,特别是影响JA信号.
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