在与败血症相关的ARDS中模拟肺内皮功能障碍,使用微生理系统
Nai-Wen Liang1, Carole Wilson2, Brooke Davis3
1Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin, USA.
Physiological reports
|July 9, 2024
概括
微生理系统 (MPS) 揭示了败血症患者的血如何损害肺内皮细胞,导致急性呼吸困扰综合征 (ARDS). 该模型为研究ARDS机制和寻找向治疗方法提供了一种新的方法.
科学领域:
- 肺部医学 肺部医学
- 关键护理医学 关键护理医学
- 生物医学工程 生物医学工程
背景情况:
- 内皮功能障碍是急性呼吸系统应急综合征 (ARDS) 严重程度和结果的关键因素.
- 由于生物差异,现有的临床前模型往往无法转化为人类疗法.
- 微生理系统 (MPS) 为研究人类肺内皮反应提供了一个有前途的平台.
研究的目的:
- 通过使用MPS. 调查败血症患者血对肺内皮细胞的影响.
- 为了确定因败血症引起的ARDS中内皮功能障碍的特定机制.
- 建立MPS作为开发有针对性的ARDS疗法的工具.
主要方法:
- 使用肺内皮微生理系统 (MPS).
- 从败血症患者和健康对照组暴露于血后,评估了内皮透性,粘附分子表达 (ICAM-1) 和细胞因子分泌 (IL-6).
- 与ARDS和没有ARDS的败血症患者之间的比较反应.
主要成果:
- 败血症血显著恶化了内皮屏障功能,导致细胞收缩和缺陷.
- 与败血症等离子体相比,与健康等离子体相比,化与败血症等离子体增加了内皮ICAM-1表达和IL-6分泌.
- 患有ARDS的败血症患者的血显示IL-6和可溶性ICAM-1水平升高.
结论:
- 肺内皮MPS可以有效地模拟与ARDS相关的败血症诱导的内皮功能障碍.
- 这个平台允许对驱动ARDS的特定机制进行审讯.
- MPS显示了在败血症患者中识别和测试ARDS新型治疗点的潜力.
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