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快速分析未经处理的食品样本,使用凝加载尖端喷雾电离质谱仪

Md Matiur Rahman¹, Shuanglong Wang², Jiaquan Xu²

¹Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang, 330013, People's Republic of China. matiurrahman@ecit.cn.

Analytical and bioanalytical chemistry

|July 9, 2024

概括

一种新的,低成本的基于尖端的电子喷雾电离质谱法 (ESI-MS) 方法使未经处理的食品样本能够进行快速,稳定和多功能分析. 该技术为全面的化学表征提供了持久的信号,克服了以前环境电离方法的局限性.

科学领域:

分析化学分析化学
食品科学食品科学食品科学
质谱测量质量谱测量

背景情况:

在全球范围内,对食品分析的快速,高效和低成本的分析方法有需求.
现有的环境电离技术,特别是电子喷雾电离 (ESI),提供快速的分子特征,但往往遭受短信号持续时间,限制全面分析.
对于食品等复杂矩阵来说,在ESI中提高稳定性和多功能性的需求至关重要.

研究的目的:

开发一种低成本,稳定和多功能的环境电离方法,用于使用质谱仪直接分析未经处理的食品样本.
为了克服传统基于ESI的食品分析中信号持续时间不足的局限性.
为了证明开发的方法的适用性,用于定量分析和粘性样本的分析.

主要方法:

使用商业上可用的一次性凝装载尖端作为低成本的发射器,用于未经处理的食品样本的直接电离.
采用基于尖端的电子喷雾电离,与质谱学 (tip-ESI-MS) 相结合.
对特定分析物 (硫胺,HMF,) 进行了定量分析,并证明了粘性样品的顺序电离.

主要成果:

实现了高信号稳定性和耐用性 (超过10分钟) 的低成本 (约. 每次运行0.10美元).
证明了较低的样品和溶剂消耗,防止尖端堵塞,操作简单,以及自动化潜力.
在真实食品样本中获得了优秀的定量结果,检测极限低 (例如,HMF的0.005μg/mL) 和高线性 (R2 ≥0.980) 的目标分析物.
成功地对未经处理的粘性食品样本进行了序列分子电离,这是传统ESI难以实现的壮举.

结论:

Tip-ESI-MS是一种高度稳定的,持久的,具有成本效益和多功能性的分析方法,用于快速化学分析未经处理的食品样本.
该方法克服了现有的环境电离技术的关键局限性,为食品分析提供了更好的性能.
这种技术由于其简单性和自动化能力,对日常食品安全监测和质量控制具有重大潜力.

关键词:

生物样本的样本是生物样本.直接分析是一种直接分析.凝装载尖端凝装载尖端纳米喷雾质谱法质谱学连续的电离. 连续的电离.

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