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使用微扫描系统进行快速抗菌敏感性测试:从阳性血液培养物中进行4小时细菌培养的性能评估

Alexander Krüger^1,2, Barbara Körber-Irrgang³, Greta Flüh³

¹Wisplinghoff Laboratories, Horbeller Straße 18-20, 50858, Cologne, Germany. a.krueger1010@googlemail.com.

Current microbiology

|July 9, 2024

概括

针对血液感染 (BSI) 的快速抗微生物敏感性测试 (AST) 是至关重要的. 使用MicroScan进行的4小时的亚培养方法证明了高精度和显著的时间节省,而不是标准的过夜检测,用于检测阴性细菌.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
诊断测试诊断测试诊断测试

背景情况:

快速抗微生物敏感性测试 (AST) 对于诊断血液感染 (BSI) 至关重要.
目前的标准AST方法通常需要一夜间化,延迟治疗决策.

研究的目的:

通过使用DxM MicroScan WalkAway系统评估AST快速4小时亚培养方法的准确性和效率.
为了比较这种快速方法与在阳性血液培养中对格拉姆阴性细菌的标准夜间 (18-24小时) 培养.

主要方法:

102个随机选择的阳性血培养 (PBC) 带有格拉姆阴性细菌被分培养.
在4小时化后,AST使用MicroScan系统进行,并在18-24小时 (标准) 后再次进行.
基本协议 (EA) 和分类协议 (CA) 为1909种药物菌株对计算.

主要成果:

与标准方法相比,4小时的亚文化方法获得了高的基本协议 (EA) 98.6%和分类协议 (CA) 97.1%.
错误率很低:小错误 (mE) 1.1%,重大错误 (ME) 0.4%,非常重大错误 (VME) 12.9%和调整后的非常重大错误 (aVME) 5.3%.

结论:

使用MicroScan的4小时亚培养方法提供了一种可靠且显著更快的方法,用于在负BSI.中检测AST.
这种快速测试策略可以加快诊断,并通过使治疗更快地进行调整来改善患者的治疗结果.

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