伊索拉姆尼丁通过激活Pi3k/Akt信号通路来改善卵细胞成熟
Xiaoya Li1, Jianyong Cheng1, Qichun Yao1
1College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, P. R. China.
Molecular nutrition & food research
|July 10, 2024
概括
饮食中的黄胺 (Isorhamnetin) 通过减少氧化应激,亡和内细胞网膜应激来增强卵细胞成熟. 这种天然化合物显示出改善卵子质量和解决女性不孕症的潜力.
科学领域:
- 生殖生物学和生物化学
- 营养学研究 营养学研究
- 细胞应激机制 细胞应激机制
背景情况:
- 伊索拉姆尼丁是一种天然存在的黄类化合物,可以在日常饮食中找到.
- 黄类药物具有多种不同的药理活动.
- 卵细胞成熟对于女性生育能力至关重要.
研究的目的:
- 为了研究异黄hamnetin对卵细胞成熟的影响.
- 阐明依索拉姆尼丁对卵细胞作用的分子机制.
主要方法:
- 卵细胞在44小时内用不同度的异胺素 (530μM) 进行了化.
- 评估极体挤出率,活性氧物种 (ROS) 水平和SOD2蛋白表达.
- 分析了线粒体自,与亡相关的蛋白质 (Bcl-2,Bax/Bcl-2,C-Casp3) 和内质网膜 (ER) 压力标志物 (CHOP,GRP78).
- 研究PI3K/Akt信号通路的激活.
主要成果:
- 伊索拉姆尼丁 (10μM) 显著增加了极体挤出率.
- 降低ROS水平和增加SOD2表达,减轻氧化应激.
- 通过调节Bcl-2,Bax/Bcl-2和C-Casp3.3来抑制亡.
- 减少了ER压力标志物 (CHOP,GRP78) 和改善了ER分布.
- 激活了PI3K/Akt信号通路.
结论:
- 伊索拉姆尼丁促进了卵细胞的成熟.
- 机制涉及减轻氧化应激,线粒体功能障碍,亡和ER压力.
- 伊索拉姆尼丁具有提高卵细胞质量和治疗女性不孕症的潜力.
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