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6 - 酸甲酸酶对于酸通路的有效运行至关重要

Léa Phégnon¹, Julien Pérochon¹, Sandrine Uttenweiler-Joseph¹

¹TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.

The FEBS journal

|July 10, 2024

概括

微生物的代谢网络是强大的. 在大肠杆菌中,细胞外途径弥补了6 - 糖核糖酶 (Pgl) 的缺失,维持了酸途径的流量,但以较低的速度.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
生物化学生物化学

背景情况:

微生物代谢网络由于基因重复和替代途径等冗余性,表现出了显著的稳定性.
酸路径 (oxPPP) 的氧化分支依赖于6-酸甲酸酶 (Pgl) 来进行6-酸甲酸的水解.
在没有Pgl的情况下,自发水解和潜在的细胞外通路被假设为维持oxPPP流量.

研究的目的:

调查细胞内非酶反应是否可以弥补大肠杆菌中Pgl的缺失.
确定Pgl在维持oxPPP流量的作用及其对中心代谢的影响.
阐明细胞外途径对Δpgl突变体酸盐合成的贡献.

主要方法:

对大肠杆菌 (Escherichia coli) 进行基因操纵,以制造 Δpgl 突变.
代谢流量分析以量化途径贡献.
细胞外水解通路的表征对于葡萄糖酸盐.

主要成果:

这项研究验证了大肠杆菌中 Δpgl 的活跃绕行通路,涉及细胞外自发水解的葡萄糖.
代谢流量分析证实,在没有Pgl的情况下,这个绕道路径占整个oxPPP流量.
细胞生长通过这种替代性,部分细胞外路线持续,但与野生类型相比,细胞生长率较低.

结论:

细胞内非酶性水解6 - 酸可诺拉克不能弥补缺少Pgl的情况.
细胞外通路维持了Pgl缺乏的大肠杆菌中的oxPPP流量,突出显示了代谢灵活性.
Pgl在确保oxPPP的高效运作和最佳细胞代谢方面发挥着至关重要的作用.

关键词:

这是NMR的NMR.葡萄糖氨酸的使用方法代谢绕道的代谢绕道代谢流动的代谢流.代谢网络是代谢网络.这是一种基酸 (phosphogluconolactone).

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