相关实验视频
Updated: Jun 21, 2025

05:51
Characterization of a Novel Human Organotypic Retinal Culture Technique
Published on: June 9, 2021
3.9K
在2型糖尿病中,骨髓系衍生型亲血管细胞的异常;对糖尿病视网膜病变的含义?
Mahnaz Shariatzadeh1, Trishika R R Binda1, Conny van Holten-Neelen2
1Department of Immunology, Erasmus University Medical Center, Rotterdam, Netherlands.
Frontiers in ophthalmology
|July 10, 2024
概括
2型糖尿病 (T2DM) 改变了亲血管细胞 (PAC),影响了他们在糖尿病视网膜病变 (DR) 中的功能. 虽然T2DM PAC显示功能变化,但DR和非DR患者之间的差异很小,这表明血管支持受损.
科学领域:
- 眼科医生 眼科 眼科
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 糖尿病视网膜病变 (DR) 是2型糖尿病 (T2DM) 的显著微血管并发症.
- 益血管性细胞 (PAC) 参与DR,但它们在T2DM中的特定异常尚未完全理解.
- 了解T2DM中的PAC表型和功能对于阐明DR病原性至关重要.
研究的目的:
- 为了评估健康对照者的PAC特征,T2DM患者有DR,T2DM患者没有DR.
- 为了确定糖尿病对PAC表型和功能的影响.
- 为了确定DR和非DRT2DM患者之间的PAC差异.
主要方法:
- PAC是由外围血液单核细胞 (PBMC) 生成的,并且具有免疫型.
- 细胞根据CD14,CD105和CD133的表达进行了分类.
- 使用体外3D内皮管形成试验来评估血管生成潜力和媒介体生产.
主要成果:
- 与对照组相比,来自T2DM患者 (有或没有DR) 的PAC显示CD16,CD105和CD31的表达较低.
- 从T2DM衍生出的PAC种群在体外增强了内皮细胞血管生成.
- 在T2DM PAC中观察到S100A8的增加和VEGF-A分泌的减少.
结论:
- 与健康对照组相比,T2DM中的PAC表现出改变的表型和功能.
- 在DR和非DR的T2DMPAC之间发现了有限的差异.
- 损伤的T2DM PAC可能导致血管支持不足和视网膜病理性新血管化.
相关概念视频
Diabetes Mellitus: Type 2 and Gestational
2.3K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
2.3K
Pathophysiology of Diabetes
917
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
917
Diabetes Mellitus: Overview and Type I Subtype
2.5K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
2.5K
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Diabetes: Symptoms, Diagnosis, and Complications
521
For most patients, experiencing several weeks of polyuria, polydipsia, fatigue, and significant weight loss may indicate the presence of diabetes. Furthermore, adults displaying the phenotypic appearance of type 2 diabetes (particularly those who are obese and not initially insulin-requiring), may have islet cell autoantibodies, suggesting autoimmune-mediated β cell destruction and a diagnosis of latent autoimmune diabetes of adults (LADA). The categorization of glucose homeostasis is...
521

