素1的表达受到hsa_circ_0055054/microRNA‐122‐5p的调节,并促进肝细胞癌的发展
Pengfei Hao1, Qi Li2, Haoliang Zhao1
1Department of General Surgery, Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, Shanxi 030032, P.R. China.
Oncology letters
|July 10, 2024
概括
异常的真1 (MUC1) 表达驱动肝细胞癌 (HCC) 的进展. 这项研究显示hsa_circ_0055054通过miR-122-5p降低MUC1的调节,抑制HCC细胞的生长和扩散.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因规则 基因规则
背景情况:
- 异常的MUC1表达与肝细胞癌 (HCC) 的预后不佳有关.
- 竞争性内源RNAs (ceRNAs) 提供了影响瘤行为的新型调节机制.
研究的目的:
- 为了研究hsa_circ_0055054和微RNA (miR) 122-5p对MUC1表达的调节作用.
- 阐明它们对HCC细胞增殖,迁移和入侵的影响.
主要方法:
- 对MUC1和hsa_circ_0055054的表达分析进行了西部抹杀和RT-qPCR.
- 细胞计数套件-8,伤口愈合,以及用于表型评估的Transwell测试.
- 生物信息学,双露西法酶记者测定和体内模型来确认circRNA-miRNA-MUC1相互作用.
主要成果:
- 在MHCC97LHC细胞中观察到高MUC1和hsa_circ_0055054表达,低miR-122-5p.
- 抑制hsa_circ_0055054或过度表达miR-122-5p降低了MUC1水平,抑制了细胞增殖,迁移和入侵.
- 在MUC1表达低的细胞中抑制miR-122-5p (由于hsa_circ_0055054敲击) 逆转了这些抑制作用.
结论:
- Hsa_circ_0055054 作为一个 ceRNA,海绵 miR-122-5p 在 HCC 上调节 MUC1 表达.
- 针对 hsa_circ_0055054/miR-122-5p/MUC1 轴为HCC提供了一个潜在的治疗策略.
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