LC-MS代谢学和分子对接方法用于识别来自Melastoma malabathricum的抗高血糖和抗氧化化合物
Oke Anandika Lestari1,2, Nurheni Sri Palupi1,3, Agus Setiyono4
1Department of Food Science and Technology, IPB University, Bogor 16680, Indonesia.
Saudi journal of biological sciences
|July 10, 2024
概括
梅拉斯托马 (Melastoma malabathricum) L. 叶片提取物含有异甲和甲等化合物,这些化合物在治疗糖尿病方面具有潜力. 这项研究科学验证了其作为抗糖尿病草药的传统用途.
科学领域:
- 药理学 药理学是指药理学的学科.
- 植物化学 植物化学
- 计算化学的计算化学
背景情况:
- Melastoma malabathricum L. (Karamunting/Senduduk) 叶在印度尼西亚传统上用于治疗糖尿病.
- 对其抗糖尿病作用负责的特定生物活性化合物的科学证据有限.
研究的目的:
- 为了确定M. malabathricum L. 叶子中具有抗高血糖和抗氧化特性的生物活性化合物.
- 通过代谢学和分子对接来验证这种植物在糖尿病管理中的传统用途.
主要方法:
- 基于液态染色学-质谱学 (LC-MS) 的叶子提取物的代谢学.
- 在体内和体内抗高血糖和体内抗氧化剂测定.
- 多变量数据分析以将生物活性与已识别的化合物相关联.
- 对抗α-葡萄糖酶,α-氨基酶和胰岛素受体的分子对接模拟.
主要成果:
- 鉴定出异二和二是α-氨基酶抑制剂;鲁丁和二是α-葡萄糖酶抑制剂.
- 奎尔素,酸,奎尔素,表素,异奎尔素和7-基氨酸与抗高血糖和抗氧化活性相关.
- 分子对接证实了抑制相互作用,在胰岛素受体上具有甲和酸之间的潜在协同作用.
结论:
- 代谢学和分子对接成功地确定并证实了M. malabathricum L. 叶中的关键抗高血糖和抗氧化化合物.
- 这项研究为M. malabathricum L.作为天然抗糖尿病剂的传统使用提供了科学验证.
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