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在糖尿病中,X-Paste通过NF-E2相关因子/HO-1信号通路改善了伤口愈合
Ming-Wei Du1,2, Xin-Lin Zhu2, Dong-Xing Zhang3
1Institute of Cardiovascular Disease, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China.
World journal of diabetes
|July 10, 2024
概括
通过激活Nrf2/HO-1通路,X-Paste (XP) 显著改善了糖尿病伤口愈合. 它的关键成分安德罗格拉福利德 (Andrographolide) 保护内皮细胞并减少炎症,为糖尿病足提供了有前途的治疗方法.
科学领域:
- 生物医学科学 生物医学科学
- 药理学 药理学 是一个学科.
- 伤口治愈研究研究 伤口治愈研究
背景情况:
- 糖尿病足 (DFU) 是糖尿病 (DM) 的严重并发症,增加截肢和死亡的风险.
- 对DFU有效的治疗策略仍然是一个关键的未满足的需求.
研究的目的:
- 调查X-Paste (XP) 对于糖尿病伤口愈合的治疗机制.
- 阐明XP的关键成分安德罗格拉福利德 (Andro) 在调节与DFU相关的细胞过程中的作用.
主要方法:
- 使用传统中医药方法制备XP,通过HPLC进行成分分析.
- 评估XP在链毒素 (STZ) 诱导的糖尿病小鼠模型中的疗效.
- 用RNA测序进行基因表达分析和分子对接以确定机制.
- 在人静脉内皮细胞 (HUVEC) 暴露于高葡萄糖 (HG) 的体外研究,包括Nrf2敲击实验.
主要成果:
- 在糖尿病小鼠中,XP显著加速了伤口愈合.
- RNA测序确定了XP处理的组织中的Nrf2上调;Andro显示出强大的Nrf2结合.
- 通过Nrf2/HO-1通路激活,安德罗减轻了HG诱导的HUVEC损伤,包括减少扩散,转移和炎症.
结论:
- 在糖尿病模型中,XP有效促进伤口愈合.
- 安德罗格拉化物是XP中的关键活性成分,激活Nrf2/HO-1通路.
- 安德罗增强内皮细胞的增殖,管体的形成,并减少炎症,这表明DFU的治疗潜力.
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