开发一种快速可靠的方法,使用LC-MS/MS在加工食品中同时检测七种食物过敏原
Akira Torii1,2, Yusuke Seki1, Ryoichi Sasano2,3
1Nisshin Seifun Group Inc., 5-3-1 Tsurugaoka, Fujimino-City, Saitama 356-8511, Japan.
Food chemistry: X
|July 10, 2024
概括
一种新的LC-MS/MS方法可以快速检测加工食品中七种常见的食物过敏原. 这种可靠的技术可确保准确的食品过敏原标签,即使在微量水平 (<1 mg/kg).
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 准确的食品过敏原标签对于消费者安全至关重要.
- 现有的检测多种食物过敏原的方法可能耗时且复杂.
- 对于加工食品,需要快速可靠的检测方法.
研究的目的:
- 开发一种快速可靠的方法,在加工食品中同时检测七种食物过敏原.
- 使用LC-MS/MS进行过敏性蛋白质的敏感量化.
- 为了简化样本准备过程用于过敏原分析.
主要方法:
- 开发一种液体染色学-双重质谱法 (LC-MS/MS) 方法.
- 用于样品制备的悬浮捕获 (S-Trap) 柱子.
- 采用在线自动化固体相提取来简化预处理.
- 分析了五个带有微量过敏原的食品样本.
主要成果:
- 同时检测小麦,白麦,牛奶,蛋,甲类动物,花生和核桃蛋白质的标记.
- 每个向蛋白质的检测值在1mg/kg以下的检测值的达到极限.
- 在复杂的加工食品矩阵中证明方法的有效性.
结论:
- 开发的LC-MS/MS方法为食品过敏原分析提供了快速可靠的方法.
- 该方法有效地选各种加工食品,以检测七种关键过敏原的存在.
- 简化协议提高了确认食品过敏原标签合规性的效率.
关键词:
检测方法 检测方法食物过敏原是一种食物过敏原.这是LC-MS/MS.类标记物是一种类标记物.加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品加工食品固体相提取方法 固体相提取方法更多相关视频
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