探索微生物和湿热对Pu-erh茶的挥发物的影响,并利用分子感官科学理解甲氧的形成机制
Tiehan Li1,2, Yuming Wei1,2, Mingxia Lu1,2
1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Food chemistry: X
|July 10, 2024
概括
在Pu-erh茶堆发酵 (PF) 期间的微生物作用会产生陈旧的香味,而潮湿的热量条件会产生梅子和甜味. 像Blastobotrys这样的特定真菌是形成芳香化合物的关键.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 堆积发酵 (PF) 对于普茶的特色香味至关重要.
- 微生物活动和湿热条件对PF气味质量的具体影响尚不清楚.
研究的目的:
- 阐明微生物和湿热作用在PF期间塑造Pu-erh茶香的作用.
- 识别关键的挥发性化合物及其形成途径,特别是甲氧.
主要方法:
- 使用固态微提取,溶剂辅助风味蒸发和气色谱质谱 (GC-MS) 进行挥发性分析.
- 采用气相色谱-嗅算法 (GC-O) 和气味活性值评估来识别芳香活性化合物.
- 进行了气味遗漏/添加实验和酸添加试验,以验证化合物的重要性和微生物贡献.
主要成果:
- 微生物的作用主要形成了陈旧的气味,而潮湿的热量条件产生了梅香和甜美的气味.
- 在184种挥发性成分中确定了20种微生物和28种湿热标记物.
- 选了22种芳香活性化合物,包括各种甲氧,与特定的真菌 (Blastobotrys,Rasamsonia,Thermomyces) 与甲氧合成正相关.
结论:
- 微生物是Pu-erh茶中甲氧合成的主要驱动因素.
- 这项研究通过分子感官科学阐明了Pu-erh茶香气挥发物的形成机制,特别是甲氧,通过分子感官科学.
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