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双站式化学传感器用于可视化OH-GSH氧化和跟踪ferroptosis诱导途径在体内

Zuzhe Kang¹, Yue Zhou¹, Yatuan Ma¹

¹College of Chemistry & Pharmacy, Northwest A&F University, Yangling, Shaanxi 712100, China.

Analytical chemistry

|July 10, 2024

概括

一种新的双位点光化学传感器OG-3能够实时监测基 (•OH) 和谷 (GSH) 以揭示铁灭途径并识别抑制剂.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
化学生物学化学生物学

背景情况:

氧化应激会破坏细胞的氧化还原平衡,影响生理过程.
细胞内基 (•OH) 和谷氨 (GSH) 是氧化应激的关键调节剂.
对OH和GSH相互作用的实时监测对于理解细胞氧化还原动力学至关重要.

研究的目的:

开发一种新的双位点光化学传感器 (OG-3),用于独立实时对OH和GSH进行成像.
为了研究OH和GSH在ferroptosis诱导途径中的作用.
评估OG-3在评估铁灭抑制剂和治疗策略方面的潜力.

主要方法:

开发OG-3,一个双位点光化学传感器,用于同时检测OH和GSH.
使用OG-3在单独的光通道中对OH和GSH进行in-situ细胞和体内成像.
监测OH-GSH氧化还原相互作用以阐明铁灭机制.

主要成果:

OG-3成功地在细胞和体内成像了外源和内源的OH和GSH,避免了交叉干扰.
该研究确定了两种不同的铁灭诱导途径:外部 (系统x_c^-抑制) 和内在 (GPX4不激活).
通过跟踪OH和GSH波动,OG-3促进了对铁灭抑制剂的评估.

结论:

OG-3提供了一个可靠的平台,用于实时现场监测OH和GSH氧化还原动力学.
这种方法阐明了铁灭菌的关键机制,并有助于发现潜在的铁灭菌抑制剂.
开发的化学传感器对推进针对铁亡的治疗策略具有前途.

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