将直肠粘膜附着的活跃微生物群与宿主免疫联系起来,揭示了它在宿主-致病性STEC O157相互作用中的作用
Zhe Pan1, Yanhong Chen1, Mi Zhou1
1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
The ISME journal
|July 10, 2024
概括
直肠-门结节微生物组影响了小牛中产生Shiga毒素的大肠杆菌O157 (STEC O157) 的殖民. 像stx2a这样的STEC O157毒性因子会改变宿主免疫力,使有益的微生物转变为机会性病原体.
科学领域:
- 兽医微生物学 兽医微生物学
- 胃肠道健康 胃肠道健康
- 食品传播病原体研究 食品传播病原体研究
背景情况:
- 直肠-门连接 (RAJ) 是牛群中第一种产生Shiga毒素的Escherichia coli (STEC) O157殖民点,促进了食物链的传播.
- 了解RAJ粘膜附着微生物组在宿主-STEC O157相互作用中的作用,对于控制病原体传播至关重要.
研究的目的:
- 为了研究RAJ粘膜附着的微生物群组成及其在小牛STEC O157感染期间对宿主免疫力的影响.
- 确定STEC O157毒性因子,特别是stx2a对宿主微生物相互作用和免疫反应的影响.
主要方法:
- 从用STEC O157菌株 (具有或没有功能性stx2a) 挑战的小牛RAJ粘膜活检的分析.
- 微生物群的概况 (多样性,拓,组合) 和粘膜体转录组分析.
- 对宿主-微生物相互作用和预测的细菌功能进行综合分析.
主要成果:
- STEC O157 stx2a生产显著改变了RAJ微生物多样性,拓和组装模式.
- 帕尼克洛斯特和加利细菌在微生物相互作用和组装中作为基石分类物种出现.
- STEC O157挑战诱导了stx2a依赖宿主免疫反应,包括B细胞和T细胞信号传递.
- 有益的微生物在挑战前保持了稳态,而像Paeniclostridium这样的机会性病原体在挑战后以stx2a依赖的方式与宿主免疫相互作用.
结论:
- 附着RAJ粘膜的微生物群在STEC O157感染动态中起着调节作用.
- 宿主-微生物相互作用可以从有益转变为机会主义,由STEC O157毒性因素 (如stx2a) 驱动.
- 针对这些宿主-微生物相互作用可能为控制牛中STEC O157传播提供新的策略.
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