植物细胞,组织和器官培养的介绍:目前的状态和前景
Víctor M Loyola-Vargas1, Neftalí Ochoa-Alejo2
1Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico. vmloyola@cicy.mx.
Methods in molecular biology (Clifton, N.J.)
|July 10, 2024
概括
植物细胞,组织和器官培养 (PCTOC) 是植物研究,微繁殖和育种的多功能工具. 这些体外技术对于基因改进,生殖质保护和商业生产有价值的植物化合物至关重要.
科学领域:
- 植物生物学 植物生物学
- 生物技术是生物技术.
- 农业科学 农业科学
背景情况:
- 植物细胞,组织和器官培养 (PCTOC) 作为基础植物科学中的实验系统.
- PCTOC使基因功能研究,研究发育过程,如胚胎生成和器官生成,并探索二次代谢产生的研究.
研究的目的:
- 突出PCTOC在基础研究和商业环境中的多样化应用.
- 强调PCTOC在植物育种,生殖细胞保护和基因工程方面的作用.
- 讨论OMIC技术对PCTOC的影响.
主要方法:
- 使用PCTOC进行基因功能证明 (过度表达/抑制).
- 应用PCTOC用于各种植物物种的植物性繁殖 (微繁殖).
- 采用PCTOC产生同卵性菌株,多卵性植物和无病个体.
主要成果:
- PCTOC促进了装饰,园艺,水果和树木物种的商业微型繁殖.
- 有效的体外植物再生协议对于通过基因工程和基因组编辑进行植物遗传改进至关重要.
- 公司利用体外PCTOC来商业化具有生物活动的植物二次代谢物.
结论:
- 对于植物科学,农业和生物技术的发展,PCTOC是不可或缺的.
- 奥米克技术的整合有望进一步增强PCTOC应用.
- PCTOC协议对于作物改进,保护和有价值化合物的生物生产至关重要.
关键词:
适应习惯的习惯化另一种文化是文化.水生物种水生物种水生物种消毒文化是消毒的文化.хлоропласт 是一种质塑料.通过DNA甲基化.危物种 危物种 危物种基因转换是一种基因转换.基因组学就是基因组学.数学模型的数学模型.药用物种 药用物种 药用物种代谢学 代谢学 代谢学微生物污染的发生微繁殖是一种微繁殖.模型植物模型植物.蛋白质组学是指蛋白质组学.原始塑料是原始塑料中的一个.复原性物种是一种反复性物种.二次代谢产物二次代谢产物实体胚胎发生体质胚胎发生.暂时的沉浸浸,即暂时的沉浸.它们是四平流体.危物种 危物种 危物种文字转录学 (Transcriptomics) 是一个学科.不常见的增长调节剂 不常见的增长调节剂相关概念视频
Plant Tissue Culture
37.7K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
37.7K
Cell Culture
17.0K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
17.0K
Stem Cell Culture
5.1K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.1K
Plant Cells and Tissues
61.0K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
61.0K
Plant Tissues
6.3K
Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...
6.3K
Plant Cell Wall
3.9K
Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
3.9K


