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转录因子ERF110通过直接调节果中的糖和醇生物合成,促进耐寒性
Madiha Khan1, Bachar Dahro1, Yue Wang1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070, China.
The Plant journal : for cell and molecular biology
|July 10, 2024
概括
乙烯反应因子 (ERF) 调节冷应力. 一种新型ERF,PtrERF110,通过直接基因调节,通过促进糖和固醇合成来提高植物的耐寒性.
科学领域:
- 植物分子生物学 植物分子生物学
- 压力生理学 压力生理学
- 生物化学 生物化学
背景情况:
- 乙烯反应因子 (ERF) 对植物对环境压力的反应至关重要,包括寒冷.
- 许多ERF在冷应激信号传递中的精确调节作用和向基因在很大程度上是未知的.
- 三叶 (Poncirus trifoliata) 是一种有价值的模型,用于研究类植物的耐寒性.
研究的目的:
- 为了识别和表征一种新的冷诱导ERF从三叶.
- 阐明这个ERF在耐寒机制中的作用.
- 为了确定参与寒冷适应的直接目标基因.
主要方法:
- 来自Poncirus trifoliata的PtrERF110的识别和特征.
- 通过果和烟草的过度表达和三叶的基因沉默 (VIGS) 来进行功能分析.
- RNA测序以识别差异表达的基因和染色体免疫沉测序 (ChIP-seq) 以确认直接目标.
主要成果:
- 具有转录激活的核蛋白PtrERF110显著提高了转基因植物的寒冷耐受性.
- 沉默PtrERF110严重损害了耐寒性,证实了它的积极作用.
- PtrERF110直接激活了PtrERD6L16,PtrSPS4和PtrUGT80B1的转录,导致糖和固醇水平的增加.
结论:
- 在植物中,PtrERF110充当了耐寒性的积极调节者.
- 该机制涉及对调节糖和固醇生物合成的基因的直接转录激活.
- 已识别的PtrERF110目标基因模块为植物寒冷适应策略提供了新的见解.
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