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细胞因子调节的细胞因子表达在Mycobacterium结核病感染的单细胞衍生-状细胞:结核病诊断的影响

Maryam Meskini¹, Mohammad Saber Zamani², Amir Amanzadeh³

¹Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; Student Research Committee, Pasteur Institute of Iran, Tehran, Iran.

|July 10, 2024

概括

结核菌菌菌的感染会改变免疫细胞中的DNA甲基化,导致像TNF-α这样的炎症性基因表达增加. 较高的MTB抗性与较低的TNF-α甲基化相关.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
传染性疾病传染性疾病

背景情况:

在Mycobacterium tuberculosis (MTB) 感染期间,研究单细胞衍生树突细胞 (mo-DCs) 中的DNA甲基化变化.
专注于促炎和抗炎基因的表观遗传调节.

研究的目的:

为了划分高分辨率的DNA甲基化变化mo-DCs对MTB感染的反应.
检查不同MTB菌株对免疫基因表达和甲基化模式的影响.

主要方法:

用各种MTB菌株 (RIFR,H37Rv,MDR,XDR) 感染mo-DCs的情况.
通过RT-PCR对亲/抗炎基因表达 (IL-6,IL-12,IFN-γ,IL-1β,TNF-α,IL-10) 的评估.
使用西方布洛特的mo-DC蛋白表达的分析.
使用MS-HRM对TNF-α和IL-10DNA甲基化的评估.

主要成果:

所有促炎基因的升级,其中TNF-α表达最高.
对抗炎症基因IL-10的下调.
在所有MTB感染群体中,TNF-α DNA甲基化显著下降 (P < 0.001).
与对照组相比,IL-10 DNA甲基化没有显著变化 (P > 0.05).

结论:

在mo-DCs中,MTB感染会诱导显著的DNA甲基化变化.
TNF-α的低甲基化与其表达的增加有关.
增加的MTB耐药性 (例如,XDR菌株) 与降低的TNF-α甲基化相关.

关键词:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.基因表达基因表达基因表达在MDR中使用MDR.结核病菌菌菌的结核病菌的结核病菌.这里是RIFR.这是XDRXDR.

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