用氧化修改的生物活性和生物降解的聚 (乳酸) -三酸支架的增材制造,用于指导骨组织修复
Samarah V Harb1,2, Elayaraja Kolanthai2, Leonardo A Pinto3
1Department of Materials Engineering (DEMa), Federal University of Sao Carlos (UFSCar), São Carlos, SP, Brazil.
Biomedical materials (Bristol, England)
|July 10, 2024
概括
这项研究开发了3D打印的聚 (乳酸) -三酸支架与氧化 (ZnO),以改善骨再生. 修改后的支架增强了细胞生长和分化,显示了骨修复应用的前景.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 整形外科的研究研究.
背景情况:
- 骨组织工程利用模仿细胞外基质的生物活性支架.
- 聚 (乳酸) - 三酸 (PLA-TCP) 复合物正在探索用于骨再生.
- 提高脚手架的生物活性和骨质生成潜力对于有效的骨修复至关重要.
研究的目的:
- 研究将氧化 (ZnO) 纳入PLA-TCP支架的效果,以提高骨组织再生.
- 优化3D打印支架的组成和制造,以提高机械性能和生物活性.
- 为了评估ZnO修饰的支架的细胞反应和骨质分化潜力.
主要方法:
- 采用不同度ZnO (1%, 5%, 10%) 的PLA-TCP复合材料制造的化丝制造 (FFF) 来制造3D支架.
- 用双螺杆挤出机与链延长器 (Joncryl) 进行编制,以使其可打印.
- 脚手架的特征包括粒子分布,孔径,机械性能,表面粗性,蛋白质吸附和生物测试 (MSC增殖,ALP活性).
主要成果:
- 脚手架显示出均的ZnO和TCP分布,可重复的300μm孔,以及合适的机械性能 (100MPa弹性模量).
- 添加ZnO增加了表面粗度 (高达241nm),增强了85%的蛋白质吸附.
- ZnO促进了介酶干细胞 (MSC) 增殖和骨质分化,性酸酶 (ALP) 活性增加了29%.
结论:
- 5%ZnO加载的PLA-TCP复合体支架显示出最佳的降解速度和增强的生物活性.
- 这些ZnO修饰的支架显示出促进骨再生和修复的巨大潜力.
- 该研究强调了将ZnO纳入用于骨组织工程的3D打印支架的好处.
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