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一个新型的Pectobacterium细菌菌体属通过准几种丹麦软腐烂分离物种来显示广泛的宿主范围

Julie Stenberg Pedersen¹, Alexander Byth Carstens¹, Magnus Mulbjerg Rothgard¹

¹Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg 1871, Denmark.

|July 10, 2024

概括

针对Pectobacterium物种的新型菌体显示出对控制土豆黑腿和软腐烂疾病的希望. 这些从丹麦田地中分离出来的菌体,为抗生素提供了潜在的生物控制替代品.

科学领域:

微生物学微生物学
植物病理学植物病理学
病毒学病毒学

背景情况:

马黑腿和软腐烂,由Pectobacteriaceae细菌引起,导致全球作物损失显著.
由于抗生素法规,目前的治疗方法有限,需要替代疾病管理策略.
菌体 (菌体) 正在成为细菌性植物疾病的有前途的生物控制剂.

研究的目的:

为了分离和描述针对土豆疾病的新型细菌菌体,针对负责土豆疾病的Pectobacterium物种.
评估新发现的菌体对丹麦乳腺细菌分离物的宿主范围和生物控制潜力.

主要方法:

在丹麦,从有症状的土豆茎和植物中分离了Pectobacterium菌株.
从有机废物中分离细菌菌体,向已识别的Pectobacterium宿主.
基因组分析,传输电子显微镜 (TEM),in silico宿主范围预测 (CRISPR-Cas数据库) 和实验性宿主范围测试.
植物遗传学分析和查 lysogeny,毒性和抗生素耐药性基因.

主要成果:

他们分离了七种新型菌体 (Ymer,Amona,Sabo,Abuela,Koroua,Taid,Pappous),代表了一个主要针对Pectobacterium brasiliense的新属.
TEM揭示了菌体Ymer的西福病毒形态;Ymer属属于具有dsDNA基因组 (39-43kb) 的Caudoviricetes.
在和实验性宿主范围测试证实了菌体感染P. brasiliense,P. polaris和P. versatile的能力,特定的菌体显示了物种内的更广泛或更窄的宿主范围.

结论:

新型Ymer属菌体在丹麦田地显示出作为生物控制剂对抗Pectobacterium诱导的土豆疾病的巨大潜力.
缺少整合酶,毒性因子和抗微生物耐药性基因,支持它们在生物控制中的安全性和有效性.
菌体表现出独特的宿主特异性,为在土豆种植中管理黑腿和软腐烂提供了有针对性的方法.

关键词:

生物控制生物控制基因组分析基因组分析菌体与宿主之间的相互作用菌体是一种菌体.软腐烂乳腺细菌科乳腺细菌

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