工程Gluconbacter oxydans具有高效的葡萄糖和比醇的联合利用,用于2-keto-L-gulonic的一步生物合成
Guang Li1, Xuyang Wang1, Weizhu Zeng2
1Engineering Research Center of Ministry of Education on Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Province Engineering Research Center of Food Synthetic Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
研究人员使用工程细菌改进了一步维生素C (甲酸) 前体合成. 这增强了葡萄糖利用和代谢途径,显著增加了2-keto-L-gulonic acid (2-KLG) 的产量.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 维生素C (甲酸) 是一个高需求的营养素,但高效的单步合成仍然具有挑战性.
- 以前使用Gluconobacter oxydans (GKLG9) 来从葡萄糖中产生2-keto-L-gulonic acid (2-KLG) 的努力受到低碳来源利用的影响.
研究的目的:
- 为了提高2KLG生产的一步发酵过程.
- 提高碳源利用率,减少Gluconobacter oxydans中副产品的形成.
主要方法:
- 识别并利用4kas基因 (4-keto-D-arabate合成酶) 来最大限度地减少碳损失和发酵的化.
- 进行了促进器工程,以促进细胞内葡萄糖运输和酸路径流量,以增加减速功率.
- 引入了D-sorbitol通路,以进一步优化2KLG生物合成.
主要成果:
- 在5L生物反应器中,在60小时内达到38.6g/L的2KLG标位.
- 达到大约46%的葡萄糖转化为-2-KLG的转化率.
- 成功降低了细胞外碳损失,并抑制了发酵的色化.
结论:
- 这项研究在单细菌,单阶段发酵中取得了重大进展,用于2-KLG生产.
- 改造的Gluconobacter oxydans菌株显示了维生素C前体合成效率和产量的提高.
- 进一步优化有望实现工业规模,可持续生产2KLG.
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