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功能性氧化还原酶Rv0547c通过重新编程宿主线粒体脂肪酸代谢来支持Mycobacterium结核病的持续性

Jayashankar Medikonda¹, Nandini Wankar¹, Suman Asalla¹

¹Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India 500046.

|July 10, 2024

概括

结核菌菌的蛋白质Rv0547c向宿主线粒体,增强脂肪酸代谢,增加线粒体的呼吸能力. 这有助于细菌在压力条件下在宿主巨细胞内持久性和生存.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
生物化学生化学

背景情况:

结核菌菌 (Mtb) 使用代谢适应和宿主操纵来生存.
了解Mtb的持续机制对于开发有效的治疗方法至关重要.

研究的目的:

调查Rv0547c的作用,一种具有预测线粒体向序列 (MTS) 的蛋白质,在Mtb持久性中的作用.
为了阐明Rv0547c如何影响宿主细胞线粒体和细菌生存.

主要方法:

研究了Rv0547c在宿主细胞线粒体中的定位和功能.
进行了比较性脂管学和分析线粒体分数.
在HEK293T细胞中评估氧气消耗率 (OCR).
在THP-1巨细胞中评估了Mtb的持久性.

主要成果:

Rv0547c的目标是独立于其预测的MTS的宿主线粒体,依赖于特定的氨酸残留物.
Rv0547c增强了线粒体膜流动性和备用呼吸能力.
观察到脂肪酸代谢发生变化,包括酸和β-氧化途径.
Rv0547c表达增加了巨细胞中的Mtb持久性和压力下的M. smegmatis存活率.

结论:

Rv0547c是第一个确定的mtb蛋白,它调节宿主线粒体代谢,特别是脂肪酸氧化.
这种调节通过增加线粒体备用呼吸能力和减轻压力来提高宿主巨细胞中的细菌存活率.
Rv0547c对Mtb的持久性和生存能力起着重要作用.

关键词:

粮农组织粮农组织粮农组织粮农组织.脂质代谢脂质代谢是一种脂质分析 (Lipid Profiling) 是一种分析方法.代谢流量是指代谢的流量.结核病菌菌菌的结核病菌的结核病菌.氧化减氧剂可以减氧.Rv0547c 在线观看

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