在感染后刺激性肠综合征中增加结肠透性的转录和代谢相关性
Ana Y Caceres Lessa1, Adam Edwinson1, Hiroki Sato2
1Division of Gastroenterology and Hepatology, Enteric Neuroscience Program, Mayo Clinic, Rochester, Minnesota.
感染后刺激性肠综合征 (PI-IBS) 与结肠透性增加和肠道代谢物改变有关,特别是在女性中. 这些发现提供了对PI-IBS病理生理学和潜在治疗点的见解.
科学领域:
- 胃肠病学 胃肠病学
- 微生物组研究 微生物组研究
- 分子生物学分子生物学
背景情况:
- 后感染性刺激性肠综合征 (PI-IBS) 是一种常见的疾病,其潜在机制尚不清楚.
- 以前的研究主要集中在流行病学方面,使生理和分子细节在很大程度上未被探索.
研究的目的:
- 调查坎皮洛巴克特诱导PI-IBS患者的生理表型,结肠转录组,便微生物组和代谢组.
- 为了确定PI-IBS患者和健康志愿者之间的特定分子和生理差异.
主要方法:
- 招募了51名罗马III坎皮洛巴克特PI-IBS患者和39名健康志愿者 (HV).
- 通过调查问卷和特定测试在体内评估肠道透性,胃肠道过境和直肠感觉.
- 分析了便样本,使用枪枪的转基因组学和非向的代谢组学; sigmoid colon 活检进行了大量的 RNA 测序.
主要成果:
- 与HV相比,PI-IBS患者的结肠透性显著增加.
- 观察到不同的基因表达和代谢物丰度,女性的显著变化.
- 便中增加的N-乙普特瑞辛和基因组胺代谢物与增加的结肠透性和特定的临床症状有关.
结论:
- 坎比洛巴克特PI-IBS的特点是结肠透率升高,与聚胺和组胺代谢的改变有关.
- 分子变化,包括基因表达和代谢物概况,在女性PI-IBS患者中更为明显.
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