来自中国的猪肠外病原体大肠杆菌的种群结构和抗生素耐药性
Xudong Li1,2, Huifeng Hu1,2,3, Yongwei Zhu1,4,5
1National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
Nature communications
|July 10, 2024
概括
猪肠外致病原体大肠杆菌 (ExPEC) 是多种多样的,并携带显著的抗生素耐药性. 这些动物菌株与人类感染有遗传联系,突出了潜在的公共卫生威胁.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 肠外致病菌大肠杆菌 (ExPEC) 对人类和动物健康构成重大威胁.
- 来自动物的ExPEC的多样性,抗生素耐药性和动物感染潜力仍然未被充分探索.
研究的目的:
- 研究来自中国的来自猪的ExPEC分离物的遗传多样性和抗生素耐药性.
- 为了确定猪ExPEC和人类起源的大肠杆菌菌株之间的遗传关系.
- 阐明抗生素耐药性基因 (ARG) 在猪ExPEC和人类病原体中的传播途径.
主要方法:
- 499个来自猪的ExPEC分离物的基因组测序和抗生素耐药性测试.
- 遗传学分析以确定菌株多样性.
- 完整基因组的基因组分析以确定ARG和传播机制.
主要成果:
- 猪ExPEC在遗传学上是多样化的 (主要是B1和A族群).
- 15个猪的ExPEC分离体显示出与人类大肠杆菌菌株的遗传联系.
- 49个菌株含有与肠道大肠杆菌病变型相关的毒素,这表明混合病变型.
- 97%的菌株具有多药耐药性,包括对关键人类抗生素的耐药性.
- 发现了普遍存在的ARG,通常与共同转移机制有关.
- 在猪ExPEC和人类起源的沙门氏菌之间观察到携带fosaA3,blaCTX-M-14和mcr-1基因的等离子体的传播.
结论:
- 猪ExPEC是多种药物耐药性的多样性储备,对人类健康构成潜在威胁.
- 猪和人类之间的遗传联系ExPEC强调了"一健康"方法的重要性.
- 在动物中监测和控制ExPEC对于减轻ARG的传播和预防人类感染至关重要.
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