克雷布结合蛋白调节可卡因和胺调节的转录表达在横向下丘脑:在奖励和强化中的影响
Namrata Pawar1, Biru B Dudhabhate2, Vaishnavi Borade1
1Department of Biotechnology, Savitribai Phule Pune University, Pune, 411 007, India.
Molecular neurobiology
|July 10, 2024
概括
可卡因和胺调节的转录 (CARTp) 对于奖励处理至关重要. CREB结合蛋白 (CBP) 在表观遗传上调节侧向下丘脑中的CART表达,驱动寻求奖励的行为.
科学领域:
- 神经科学是一个神经科学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 神经CARTp在奖励处理中发挥着关键作用.
- 内自刺激 (ICSS) 在横向下丘脑 (LH) - 中间前脑束 (MFB) 中调节CART.
- 在ICSS期间驱动CART上调的机制尚未完全理解.
研究的目的:
- 研究表观遗传酶CREB结合蛋白 (CBP) 在ICSS期间调节CART表达中的作用.
- 为了确定CBP介导的激素乙化是否影响CART基因表达和奖励行为.
主要方法:
- 大鼠接受了ICSS调节,用LH-MFB电极植入.
- 将CBP siRNA输送到LH-MFB以降低CBP水平.
- 染色体免疫沉 (ChIP) 试验评估了对CART促进体的CBP结合和基因素乙化 (H3K9ac).
- 测量了杆压力活性和CART表达 (mRNA和).
主要成果:
- ICSS增加了CART,CBP和pCREB水平,以及对CART促进体的增强CBP结合和H3K9ac.
- CBP siRNA输注减少了杆压力,CBP水平,基因素乙化和CART表达.
- 同时输注CARTp逆转了CBPsiRNA对自我刺激的影响.
结论:
- 在LH中CBP介导的基因素乙化与CART表达有因果关系.
- 由CBP监管的CART表达驱动了奖励寻求行为的积极强化,例如按.
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