相关实验视频

Updated: Jun 7, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

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林卡特拉:双循环方法来放大RNA用于从甲固定型胺嵌入组织中的转录组分析.

Poorna Manasa Bhamidimarri1, Laila Salameh2, Amena Mahdami1

  • 1Research Institute for Medical and Health Sciences, University of Sharjah, Sharjah, United Arab Emirates.

Heliyon
|July 11, 2024
PubMed
概括

现在可以使用新的LINCATRA方法分析Formalin固态抛嵌入 (FFPE) 组织RNA进行全转录组分析 (WTA). 这种技术可以放大FFPE样本中降解的RNA,从而为疾病研究提供准确的基因表达分析.

关键词:
退化了 退化了 退化了嵌入式法固定的抛.线性放大线性放大线性放大线在RNA-Seqq.转录组分析 转录组分析

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Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens12:24

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Overview
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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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