相关实验视频

Updated: Jun 21, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

概述m6A甲基化修饰与非编码RNA之间的相互作用及其对瘤细胞的影响

Zheng Dou¹, Xiao-Ting Ma¹, Mei-Na Piao¹

¹Department of Radiation Oncology, The Affiliated Hospital of Soochow University, Suzhou, China.

Translational cancer research

|July 11, 2024

概括

N6-甲基氨酸 (m6A) 修改调节基因表达并影响癌症的发展. 本综述探讨了m6A如何与非编码RNA (ncRNA) 相互作用,从而影响瘤进展.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
癌症研究癌症研究

背景情况:

N6-甲基氨酸 (m6A) 是一种普遍存在的RNA修饰,影响mRNA拼接,稳定性和翻译.
m6A调节器 (写字器,擦拭器,阅读器) 控制m6A水平并影响细胞过程,包括与癌症相关的基因表达.
非编码RNAs (ncRNAs) 像miRNAs,lncRNAs和circRNAs在癌症中作为瘤基因或瘤抑制剂发挥着至关重要的作用.

研究的目的:

审查m6A在ncRNAs中的重要调节作用.
为了阐明人类恶性瘤中m6A和ncRNA之间的相互作用.
提供m6A调节因子和ncRNAs在癌症中的相互作用的概述.

主要方法:

对癌症中m6A和ncRNAs当前研究的文献综述.
对研究研究m6A对ncRNAs的调节机制的分析.
检查ncRNAs如何调节m6A相关的酶和过程.

主要成果:

越来越多地认识到m6A修饰在调节ncRNAs中的作用.
ncRNAs影响m6A相关酶的稳定性,拼接和翻译.
m6A和ncRNAs之间的复杂交叉对瘤开始和进展做出了重大贡献.

结论:

m6A和ncRNA之间的相互作用是人类癌症发展的关键因素.
了解这些相互作用为癌症治疗提供了潜在的治疗点.
对m6A-ncRNA网络的进一步研究对于推进癌症生物学和治疗至关重要.

关键词:

N6-甲基氨酸甲基化 (m6A甲基化)m6A 监管因素监管因素非编码RNA (ncRNA) 是一种非编码RNA.瘤是一个瘤.

更多相关视频

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Chromatin Structure Regulates pre-mRNA Processing

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...