对Schizosaccharomyces pombe分离启动网络组件的温度敏感等位基因的表征
Lesley A Turner1, Alaina H Willet1, Kathleen L Gould1
1Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, US.
microPublication biology
|July 11, 2024
概括
研究人员在四个Schizosaccharomyces pombe分离启动网络 (SIN) 组件中描述了新的温度敏感突变. 这些突变物增强了研究细胞分裂和细胞运动信号通路的可用工具.
科学领域:
- 细胞生物学 细胞生物学
- 分子遗传学 分子遗传学
- 微生物学 微生物学
背景情况:
- 在 * Schizosaccharomyces pombe * 中,隔离启动网络 (SIN) 对于调节细胞动力学和细胞隔离至关重要.
- SIN信号传递涉及一个由一个小的GTPase启动的蛋白激酶级联,并由一个两组分的GTPase激活蛋白 (GAP) 调节.
- 关键的SIN组件以细胞周期依赖的方式定位到螺旋杆体.
研究的目的:
- 在四个核心SIN组件中描述先前分离的温度敏感突变:cdc14,cdc16,sid1和sid2.
- 为了确定这些等位基因内的特定突变.
- 评估这些新突变物种在不同温度下的生长表型.
主要方法:
- 在 *Schizosaccharomyces pombe* 中隔离和描述温度敏感突变物.
- 在*cdc14*,*cdc16*,*sid1*和*sid2*等位基因中确定特定的点突变.
- 在不同温度下对新突变异位基因与已知的突变异位基因进行比较生长分析.
主要成果:
- 在 *cdc14*, *cdc16*, *sid1* 和 *sid2* 温度敏感等位基因中发现了特定的突变.
- 分析了这些新特征突变的生长特征,并与现有的等位基因进行了比较.
- 这项研究成功地扩大了对特征SIN突变的收集.
结论:
- 这些温度敏感突变体的表征为研究人员提供了宝贵的新工具.
- 这些扩展的资源将有助于进一步研究SIN信号的机制.
- 这些发现有助于更深入地了解裂变酵母细胞循环调节和细胞动力学.
相关概念视频
Septins
1.8K
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
1.8K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Yeast Signaling
14.6K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.6K
Background and Environment Affect Phenotype
6.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.5K


