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miR-126a-5p通过向ADAMTS-4来抑制H1N1诱导的炎症和肺纤维细胞中的矩阵蛋白酶分泌

Fang Fang¹, Borong Wang¹, Xiang Lu¹

¹The 2nd department of Critical Care Medicine, Xi'an Chest Hospital, Hangtian Avenue East Section, Chang'an District, Xi'an, Shaanxi, 710100, China.

Archives of virology

|July 11, 2024

概括

微RNA-126a-5p (miR-126a-5p) 通过抑制ADAMTS-4表达来保护免受H1N1流感病毒引起的肺损伤. 抑制miR-126a-5p可降低肺纤维细胞中的炎症,亡和病毒复制.

科学领域:

肺部病理学肺部病理学
病毒学病毒学
分子生物学分子生物学

背景情况:

艾达姆斯-4 (一种具有血栓蛋白因子4的分解蛋白和金属蛋白酶) 涉及到肺损伤.
微RNA-126a-5p (miR-126a-5p) 在其他条件下调节ADAMTS-4表达.

研究的目的:

研究miR-126a-5p/ADAMTS-4轴在H1N1流感病毒引起的肺损伤中的作用.
为了确定miR-126a-5p是否可以减轻H1N1引起的肺损伤.

主要方法:

肺部纤维细胞感染了H1N1流感病毒.
评估了miR-126a-5p和ADAMTS-4的表达,细胞活力 (CCK-8),炎症因子 (ELISA),细胞亡和病毒复制.
使用双 luciferase 试验验证了 miR-126a-5p 和 ADAMTS-4 的调节关系.

主要成果:

H1N1感染降低了纤维细胞活力,抑制了miR-126a-5p,并增加了ADAMTS-4表达.
过度表达miR-126a-5p减弱了炎症,亡,矩阵蛋白酶分泌和病毒复制.
miR-126a-5p直接向并抑制ADAMTS-4mRNA,而ADAMTS-4过度表达逆转了miR-126a-5p的保护作用.

结论:

miR-126a-5p对H1N1流感病毒引起的肺损伤起着保护作用.
通过向ADAMTS-4,对miR-126a-5p的升级抑制了亡,炎症因素,矩阵蛋白酶分泌和肺纤维细胞中的病毒复制.

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