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Updated: Jun 21, 2025

High-throughput Gene Tagging in Trypanosoma brucei

High-throughput Gene Tagging in Trypanosoma brucei

Published on: August 12, 2016

生命阶段特定的多A) 位点选择受Trypanosoma brucei DRBD18 的调节

Jonathan E Bard^1,2, Brianna L Tylec³, Ashutosh P Dubey³

¹Genomics and Bioinformatics Core, University at Buffalo School of Medicine and Biomedical Sciences, Buffalo, NY 14203.

Proceedings of the National Academy of Sciences of the United States of America

|July 11, 2024

概括

RNA结合蛋白DRBD18通过调节基因表达和3'未翻译区域 (3'UTR) 长度来控制Trypanosoma brucei的发育,从而影响寄生虫的分化.

科学领域:

分子生物学分子生物学
寄生虫学的寄生虫学
遗传学遗传学是一个

背景情况:

虫体表现出复杂的生命周期阶段,需要精确的基因调节.
转录控制是有限的;通过RNA结合蛋白 (RBPs) 的转录后调节对于差异化至关重要.
DRBD18是一种已知的T. brucei. 的多功能RBP.

研究的目的:

研究DRBD18在T. brucei基因调节和3'UTR长度中的作用.
用单细胞RNAseq.来描述DRBD18耗尽时的转录组变化.
确定DRBD18对替代多化和发育控制的影响.

主要方法:

单细胞RNA测序 (scRNAseq) 用于分析转录组变化和3'UTR长度.
通过RNA免疫沉 (RIP) 来确认DRBD18与标转录的直接相互作用.
分析DRBD18 knockdown与野生类型细胞中的多基化模式.

主要成果:

由于DRBD18的枯竭导致转录基因特征的改变,包括在前循环阶段上调调节形和元循环形式的转录.
DRBD18调节了超过1500个转录的3'UTR长度,有利于远端多基化站点的使用.
在DRBD18中多基化模式类似于血流中形的多基化模式,这表明DRBD18在发育中的多基 (A) 位点选择中的作用.

结论:

DRBD18在通过控制替代多基化来调节T. brucei发育方面发挥着至关重要的作用.
替代性多氨基化是T. brucei.发育基因调节的关键机制.
DRBD18直接影响特定发育转录的多样性 (A) 站点选择.

关键词:

这是一种RNA结合蛋白.替代性的多基化.动态塑化物动态塑化物生命周期生命周期试巴诺基因组的三种类型

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