2020-2021年塞内加尔的SARS-CoV-2基因组流行病学
Adjiratou Aissatou Ba1, Romain Coppée2, Assane Dieng1
1Laboratoire Bactériologie-Virologie CHNU Aristide le Dantec, Dakar, Sénégal.
Journal of infection in developing countries
|July 11, 2024
概括
塞内加尔塞内加尔塞内加尔塞内加尔塞内加尔塞内加尔塞内加尔
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 流行病学 流行病学
背景情况:
- 塞内加尔的COVID-19分子诊断是常见的,但基因组监测在公共部门是有限的.
- 这项研究分享了塞内加尔在COVID-19大流行期间公共实验室的经验.
- 它描述了2020年和2021年流通的SARS-CoV-2变种的分布.
研究的目的:
- 记录公共部门实验室对塞内加尔COVID-19流行病的反应.
- 描述塞内加尔的SARS-CoV-2变种的基因组多样性和分布.
- 提供关于基因组监测在资源有限的环境中的挑战和成功的见解.
主要方法:
- 从2020年7月到2021年12月,从旅行者和症状患者收集了鼻样本.
- 执行了SARS-CoV-2 qRT-PCR和使用纳米孔技术进行全基因组测序 (WGS) 的CT ≤30的选择样本.
- 开发了用于空间和时间变异分布分析的内部脚本,结合了GISAID数据.
主要成果:
- 在8207名查人员中,970人检测出SARS-CoV-2阳性.
- 在133个样本上进行了全基因组测序,在2020年确定了20A和20B系.
- 在2021年,Delta变种 (AY34和AY.34.1) 主导,占测序样本的22%.
结论:
- 建立了常规的COVID-19诊断,但基因组特征面临挑战.
- 在塞内加尔观察到的基因组多样性反映了早期流行病浪潮期间的全球趋势.
- 该研究强调了公共部门参与传染病基因组监测的重要性.
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