在成年雌性小鼠中,TRPV1和乳腺细胞参与了重复变异性压力诱导的尿膀功能障碍
Amanda B Sidwell1, Beatrice M Girard1, Susan E Campbell1
1Department of Neurological SciencesThe Larner College of Medicine, University of VermontBurlingtonVermontUnited States.
American journal of physiology. Renal physiology
|July 11, 2024
概括
压力会通过短暂受体潜在化物1 (TRPV1) 通道和巨细胞加剧膀疼痛综合征 (IC/BPS). 向TRPV1和巨细胞可以缓解压力诱导的尿功能障碍.
科学领域:
- 神经科学是一个神经科学.
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 免疫学 免疫学 免疫学
背景情况:
- 间歇性囊炎/膀疼痛综合征 (IC/BPS) 病因是多因素的,有压力加剧症状.
- 暂时受体潜在化物1 (TRPV1) 通道和巨细胞与IC/BPS有关.
- 将压力与膀功能障碍联系在一起的机制需要阐明.
研究的目的:
- 为了研究TRPV1和巨细胞在压力诱导的膀功能障碍中的作用.
- 为了确定TRPV1和乳腺细胞是否有助于增加压力后排泄频率.
主要方法:
- 在雌性野生型 (WT),TRPV1淘汰 (KO) 和乳腺细胞缺乏的小鼠中使用了重复变异应激 (RVS) 模型.
- 在WT小鼠中使用了TRPV1抗剂撒平 (CPZ).
- 测量了血清皮质,类似焦虑的行为,以及空白频率.
- 评估TRPV1蛋白质表达在脊髓和背部根 (DRG) 中.
主要成果:
- 在WT小鼠中,RVS增加了皮质和空白频率.
- 在WT小鼠中,CPZ治疗拯救了RVS诱导的膀功能障碍.
- 在TRPV1 KO和乳腺细胞缺乏的小鼠中,没有RVS诱导的排泄频率或皮质激素的增加.
- RVS在特定的脊髓段和DRG中增加了TRPV1的表达.
结论:
- TRPV1和巨细胞是导致压力诱导的膀功能障碍的关键因素.
- 准TRPV1和巨细胞可能为IC/BPS提供治疗策略.
- 这些发现突出了将压力与膀过敏相关联的潜在途径.
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