转录因子PagLBD4抑制了Populus中的细胞分化和二次细胞壁生物合成
Ying Guo1, Lijuan Yao1, Xiaoman Chen1
1State Forestry and Grassland Administration Key Laboratory of Silviculture in downstream areas of the Yellow River, College of Forestry, Shandong Agricultural University, Taian, 271018, China.
Plant physiology and biochemistry : PPB
|July 11, 2024
概括
侧面器官边界域 (LBD) 转录因子调节植物的发育. 在Populus中的PagLBD4抑制了二次体和细胞壁的形成,影响了植物的生长.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物开发 植物开发
- 树木形成的树木形成.
背景情况:
- 横向器官边界域 (LBD) 转录因子对植物生长和发育至关重要.
- 了解特定LBD基因的作用对于阐明木质植物的调节网络至关重要.
研究的目的:
- 为了功能性地描述Populus (Populus alba × Populus glandulosa) 中的PagLBD4基因.
- 调查PagLBD4在调节二次体分化和二次细胞壁 (SCW) 生物合成中的作用.
主要方法:
- 过度表达和CRISPR/Cas9介导的PagLBD4在Populus中的淘汰.
- 使用RNA测序 (RNA-seq) 进行基因表达分析.
- 通过DNA亲和力净化,然后进行测序 (DAP-seq) 来识别直接目标基因.
主要成果:
- PagLBD4过度表达抑制了二次体分化和SCW沉积.
- PagLBD4淘汰赛增强了二次体分化和SCW沉积.
- 在过度表达PagLBD4的植物中,SCW生物合成途径受到下调调节,而在淘汰植物中受到上调调节.
- 鉴定了PagLBD4的263个直接向基因,包括SCW生物合成的关键调节者.
结论:
- 在Populus中,PagLBD4充当了二次体分化和SCW生物合成的抑制剂.
- PagLBD4在调节木材形成和整体植物生长方面发挥着重要作用.
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