相关实验视频

Updated: Jun 21, 2025

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Published on: September 20, 2024

一个编码9氨酸缩酶的新型OsGST基因负面调节了大米中的积累

Rui Guo¹, Su Li², Yong Qiang Gao²

¹State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China; School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221116, China.

Journal of hazardous materials

|July 11, 2024

概括

这项研究表明,大米中的新型OsGST基因在控制 (Cd) 积累和增强植物耐药性方面发挥着至关重要的作用. 过度表达OsGST会提高Cd耐受性,而缺少它会增加Cd水平和灵敏度.

科学领域:

植物生物学植物生物学
环境毒理学环境毒理学
分子遗传学分子遗传学

背景情况:

大米中 (Cd) 污染对人类健康构成重大风险,原因是在食物链中的积累.
谷氨S转化酶 (GSTs) 是关键的酶,通过与谷氨 (GSH) 结合,参与排毒危险化合物,包括Cd等重金属.

研究的目的:

为了研究一种新型大米氨酸S转移酶 (OsGST) 基因在应对 (Cd) 压力时的功能.
阐明OSGST在调节大米植物中Cd积累和耐受性的作用.

主要方法:

利用CRISPR/Cas9基因编辑来创建OSGST删除突变大米系.
产生的转基因大米系过度表达了OsGST基因.
分析了Cd积累,Cd诱导的过敏表型,活性氧物种 (ROS) 水平和与抗氧化活性相关的基因表达.

主要成果:

暴露于CD诱导了新型OsGST基因及其蛋白质的表达,这些蛋白质定位在细胞核和细胞质中.
OsGST删除突变体显示了增加的Cd积累和过敏表型.
过度表达OsGST提高了米对Cd的耐受性,并减少了Cd的积累.
OsGST突变体表现出较高的ROS水平和较高的GSH度,与抗氧化剂相关基因的下调表达.

结论:

在控制Cd积累和增强大米中Cd耐药性方面,OsGST起着至关重要的作用.
在Cd压力下,OsGST蛋白通过维持谷氨 (GSH) 和氧化还原平衡而起作用.
针对OsGST提供了一个潜在的策略,用于开发降低Cd吸收的米品种.

关键词:

抗氧化剂是一种抗氧化剂.Cd Cd Cd Cd 在线播放这就是OsGST.这就是ROSOS ROS.大米大米大米大米大米

更多相关视频

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Geomagnetic Field Gmf and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Published on: November 30, 2015

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Activation and Inactivation of G Proteins

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...