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Updated: Jun 21, 2025

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

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阿拉比多普西斯DDM1的染色体重塑活动的分子和结构基础

Akihisa Osakabe1,2, Yoshimasa Takizawa3, Naoki Horikoshi3

  • 1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan. akihisa-osakabe@g.ecc.u-tokyo.ac.jp.

Nature communications
|July 11, 2024
PubMed
概括

在Arabidopsis thaliana中,Histon H2A.W 阻止了转子子体的进入. DDM1染色体重塑器增加了H2A.W核细胞组DNA的灵活性,使转子子的表观遗传沉默成为可能.

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相关概念视频

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
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Spreading of Chromatin Modifications

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Writers
The writer...
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The chromatin structure, especially...
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Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
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