开启蛋白质开关,用于控制细胞中的活性蛋白结合
Unyime M Effiong1, Hannah Khairandish1, Isabela Ramirez-Velez1
1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA.
Nature communications
|July 11, 2024
概括
研究人员开发了可控制的动氨酸结合开关工具 (CAST),以精确地操纵细胞内的丝状动氨酸 (F-actin). 这些遗传编码工具可以对F-actin进行局部控制,从而推进合成生物学应用.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 合成生物学 合成生物学
背景情况:
- 丝状活性蛋白 (F-actin) 对于细胞功能至关重要,由许多活性蛋白结合蛋白 (ABP) 组成.
- 研究个别的ABP和细胞下控制actin结合是具有挑战性的.
- 为合成生物学利用F-actin细胞骨需要新的操纵工具.
研究的目的:
- 开发一种用于外部控制蛋白质-actin相互作用的新系统.
- 为了使F-actin细胞骨的局部化和刺激反应性操纵.
- 探索合成生物学中的应用,以控制细胞和组织行为.
主要方法:
- 可控制的动氨酸结合开关工具 (CAST) 的设计和工程.
- 开发可响应各种外部刺激的CAST,具有可调节的动力学.
- 对CAST进行基因编码,以便将其插入原生蛋白质和工程结构中.
主要成果:
- 证明了CAST的外部可控制的活性蛋白结合行为.
- 在F-actin协会上实现了正交和多重控制.
- 通过将CAST插入本地蛋白质,展示了F-actin的局部控制.
- 将工程CAST转化为结构,以影响细胞和组织的形状和行为.
结论:
- CAST提供了一个强大的,基因编码的平台,用于精确,刺激-响应控制F-actin.
- 这项技术克服了研究ABP和操纵actin细胞骨架的局限性.
- CAST为合成生物学,细胞工程和理解F-actin动态开辟了新的途径.
相关概念视频
Actin Polymerization and Cell Motility
5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
Actin Filament Depolymerization
3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
In F-actin, the ADF/cofilin proteins...
3.1K
Generation of Straight or Branched Actin Filaments
2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Introduction to Actin
5.1K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
5.1K
The Role of Actin and Myosin in Non-muscle Cells
3.4K
Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They are held...
3.4K
Formation of Higher-order Actin Filaments
3.0K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
The high-order actin...
3.0K


